Identification of Arcanobacterium phocae isolated from fur animals by phenotypic properties, by MALDI-TOF MS analysis and by detection of phocaelysin encoding gene phl as probable novel target
| Autor: | Mazen Alssahen, Amir Abdulmawjood, Markus Timke, Jörn-Peter Wickhorst, André Becker, Ellen Prenger-Berninghoff, Osama Sammra, Mirja Raunio Saarnisto, Christoph Lämmler, Abdulwahed Ahmed Hassan |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genotype 030106 microbiology ved/biology.organism_classification_rank.species Loop-mediated isothermal amplification Foxes Arcanobacterium haemolyticum Microbiology Arcanobacterium phocae 03 medical and health sciences Bacterial Proteins RNA Ribosomal 16S Trueperella pyogenes Animals Ribosomal DNA Gene Finland Phylogeny General Veterinary biology ved/biology General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA Arcanobacterium Matrix-assisted laser desorption/ionization Phenotype Mink Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Actinomycetales Infections |
| Zdroj: | Veterinary microbiology. 216 |
| ISSN: | 1873-2542 |
| Popis: | In the present study 12 Arcanobacterium phocae strains isolated from fur animals in Finland, including foxes, minks and Finnraccoons, could successfully be identified phenotypically, by matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and phocaelysin (PHL) encoding gene phl. The PHL of all 12 A. phocae strains in the present study and reference strains A. phocae DSM 10002T and A. phocae DSM 10003 displayed, as typical members of the cholesterol dependent cytolysin-group of toxins, the variant undecapeptide sequence EATGLAWDPWW which appeared to be most closely related to arcanolysin of Arcanobacterium haemolyticum and pyolysin of Trueperella pyogenes. In addition, gene phl could be determined with a newly designed loop-mediated isothermal amplification (LAMP) assay. The detection of mass spectra by MALDI-TOF MS and the LAMP assay based on gene phl might help to reliably identify A. phocae in future and also elucidate the role this species plays in infections of fur animals. |
| Databáze: | OpenAIRE |
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