Development of an ELISA using anti-idiotypic antibody for diagnosis of opisthorchiasis
| Autor: | Sergey N Borovikov, Aitbay Bulashev, Saule Z Eskendirova, Shynar S Serikova, Zhanbolat A Suranshiev, Vladimir Kiyan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.drug_class 030231 tropical medicine Enzyme-Linked Immunosorbent Assay Biology Monoclonal antibody Opisthorchiasis Sensitivity and Specificity Epitope 03 medical and health sciences 0302 clinical medicine Antigen Blocking antibody medicine Animals Humans Opisthorchis felineus Opisthorchis Antibody titer biology.organism_classification Virology Molecular biology Antibodies Anti-Idiotypic 030104 developmental biology biology.protein Parasitology Paratope Antibody |
| Zdroj: | Folia Parasitologica. 63 |
| ISSN: | 1803-6465 0015-5683 |
| DOI: | 10.14411/fp.2016.025 |
| Popis: | Monoclonal antibody specific for an epitope of cretory-secretory antigen protein of Opisthorchis felineus (Rivolta, 1884) (Trematoda: Opisthorchiidae) with a molecular weight of 28 kDa was used in a sandwich enzyme-linked immunosorbent assay (ELISA) for immobilisation of liver fluke specific antigen to the solid phase. Examination of human sera by this ELISA compared with commercial assays demonstrated that the monoclonal antibody epitope is located within this significant parasite protein. Anti-idiotypic antibody specific for the paratope of this monoclonal antibody was obtained by a hybridoma technique. Mimicking an epitope of excretory-secretory antigen of O. felineus, it had the capacity to bind specific antibody and elicit an antibody response. The value of anti-idiotypic antibody as a substitute for the liver fluke antigen was tested by ELISA using serum samples of infected dogs. Anti-idiotypic antibody proved to be of value in both an indirect-ELISA and a competitive-ELISA for diagnosis of opisthorchiasis. Mature trematodes were isolated from all infected animals. The faecal egg counts were negative in dogs with a relatively small number of parasites, despite finding antibodies in serum by ELISA. Substitution of parasite antigen with anti-idiotype avoids the use of experimental animals and also reduces time-consuming steps of antigen preparation. |
| Databáze: | OpenAIRE |
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