Composition and Antimicrobial Activity of the Skin Peptidome of Russian Brown Frog Rana temporaria
| Autor: | Egor Vorontsov, Roman A. Zubarev, Vladimir Gorshkov, Osmo Hänninen, Elias Hakalehto, Albert T. Lebedev, Tatyana Yu. Samgina |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Salmonella typhimurium Bodily Secretions Staphylococcus aureus Salmonella Molecular Sequence Data Rana temporaria Peptide Bradykinin medicine.disease_cause Biochemistry Amphibian Proteins Mass Spectrometry Rana Microbiology Species Specificity medicine Animals Nanotechnology Amino Acid Sequence Peptide sequence Chromatography High Pressure Liquid Skin chemistry.chemical_classification biology General Chemistry biology.organism_classification Antimicrobial Anti-Bacterial Agents chemistry Salmonella enterica Peptides Antibacterial activity Antimicrobial Cationic Peptides |
| Zdroj: | Journal of Proteome Research. 11:6213-6222 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr300890m |
| Popis: | A nano-HPLC-ESI-OrbiTrap study involving HCD and ETD spectra has been carried out to clarify the composition of the skin peptidome of brown Russian frogs Rana temporaria. This approach allowed determinantion of 76 individual peptides, increasing 3-fold the identified portion of the peptidome in comparison to that obtained earlier with FTICR MS. A search for the new bradykinin related peptides (BRPs) was carried out by reconstructing mass chromatograms based on the ion current of characteristic b- and y-ions. Several peptides were reported in the secretion of R. temporaria for the first time. The overall antibacterial activity of the skin secretion in general and of one individual peptide (Brevinin 1Tb) was determined using PMEU Spectrion (Portable Microbe Enrichment Unit) technology. The inhibitory effects of these peptides on Staphylococcus aureus and Salmonella enterica Serovar typhimutium were equal in scale to that reported for some antibiotics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|