Stimulation of Single Isolated Adult Ventricular Myocytes within a Low Volume Using a Planar Microelectrode Array
Autor: | Jon Cooper, Norbert Klauke, Godfrey L. Smith |
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Rok vydání: | 2003 |
Předmět: |
Heart Ventricles
Silicones Biophysics Stimulation Contractility chemistry.chemical_compound Muscles and Contractility Extracellular fluid Myocyte Animals Humans Mineral Oil Myocytes Cardiac Dimethylpolysiloxanes Electrodes Microscopy Confocal Polydimethylsiloxane Chemistry Myocardium Multielectrode array Anatomy Electrophysiology Electrode Calcium Rabbits |
Zdroj: | ResearcherID |
ISSN: | 0006-3495 |
DOI: | 10.1016/s0006-3495(03)74606-2 |
Popis: | Microchannels (40- microm wide, 10- microm high, 10-mm long, 70- microm pitch) were patterned in the silicone elastomer, polydimethylsiloxane on a microscope coverslip base. Integrated within each microchamber were individually addressable stimulation electrodes (40- microm wide, 20- microm long, 100-nm thick) and a common central pseudo-reference electrode (60- microm wide, 500- microm long, 100-nm thick). Isolated rabbit ventricular myocytes were introduced into the chamber by micropipetting and subsequently capped with a layer of mineral oil, thus creating limited volumes of saline around individual myocytes that could be varied from 5 nL to 100 pL. Excitation contraction coupling was studied by monitoring myocyte shortening and intracellular Ca(2+) transients (using Fluo-3 fluorescence). The amplitude of stimulated myocyte shortening and Ca(2+) transients remained constant for 90 min in the larger volume (5 nL) configuration, although the shortening (but not the Ca(2+) transient) amplitude gradually decreased to 20% of control within 60 min in the low volume (100 pL) arrangement. These studies indicate a lower limit for the extracellular volume required to stimulate isolated adult cardiac myocytes. Whereas this arrangement could be used to create a screening assay for drugs, individual microchannels (100 pL) can also be used to study the effects of limited extracellular volume on the contractility of single cardiac myocytes. |
Databáze: | OpenAIRE |
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