Optimization and Validation of a Fluorescence Polarization Immunoassay for Rapid Detection of T-2 and HT-2 Toxins in Cereals and Cereal-Based Products
| Autor: | Michele Suman, Stefania Valenzano, Vincenzo Lippolis, Michelangelo Pascale, Sandro Zanardi, Marina Cortese, Anna Chiara Raffaella Porricelli |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
T-2 and HT-2 toxins
01 natural sciences Applied Microbiology and Biotechnology Rapid detection Fluorescence polarization immunoassay Oats Analytical Chemistry Crispbread Rye 0404 agricultural biotechnology food Barley medicine Safety Risk Reliability and Quality Detection limit Chromatography medicine.diagnostic_test Chemistry 010401 analytical chemistry 04 agricultural and veterinary sciences Contamination 040401 food science food.food Quantitative determination 0104 chemical sciences Immunoassay Cereal-based products Safety Research Food Science |
| Zdroj: | Food analytical methods 9 (2016): 3310–3318. doi:10.1007/s12161-016-0527-1 info:cnr-pdr/source/autori:Porricelli, Anna Chiara R.; Lippolis, Vincenzo; Valenzano, Stefania; Cortese, Marina; Suman, Michele; Zanardi, Sandro; Pascale, Michelangelo/titolo:Optimization and Validation of a Fluorescence Polarization Immunoassay for Rapid Detection of T-2 and HT-2 Toxins in Cereals and Cereal-Based Products/doi:10.1007%2Fs12161-016-0527-1/rivista:Food analytical methods/anno:2016/pagina_da:3310/pagina_a:3318/intervallo_pagine:3310–3318/volume:9 |
| DOI: | 10.1007/s12161-016-0527-1 |
| Popis: | A fluorescence polarization (FP) immunoassay has been optimized and validated for rapid quantification of T-2 and HT-2 toxins in both unprocessed cereals, including oats, barley and rye, and cereal-based products for direct human consumption, such as oat flakes, oats crispbread and pasta. Samples were extracted with 90 % methanol, and the extract was filtered and diluted with water or sodium chloride solution prior to the FP immunoassay. Overall mean recoveries from spiked oats, rye, barley, oat flakes, oats crispbread and pasta ranged from 101 to 107 %, with relative standard deviations lower than 7 %. Limits of detection (LODs) of the FP immunoassay were 70 mu g/kg for oats, 40 mu g/kg for oat flakes and barley, 25 mu g/kg for pasta and 20 mu g/kg for rye and oats crispbread. The trueness of the immunoassay was assessed by using two oat and oat flake reference materials for T-2 and HT-2 toxins, showing good accuracy and precision. Good correlations (r > 0.953) were observed between T-2 and HT-2 toxin contents in naturally and artificially contaminated samples determined by both FP immunoassay and ultra-high-performance liquid chromatography (UHPLC) with immunoaffinity column cleanup used as reference method. These results, combined with rapidity and simplicity of the assay, show that the optimized assay is suitable for high-throughput screening, as well as for reliable quantitative determination of T-2 and HT-2 toxins in cereals and cereal-based products. |
| Databáze: | OpenAIRE |
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