Comparative and phylogenetic analysis of zinc transporter genes/proteins in plants
| Autor: | Ertuğrul Filiz, Recep Vatansever, Ibrahim Ilker Özyiğit |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Protein family Physiology Protein domain Biology 01 natural sciences Microbiology Homology (biology) 03 medical and health sciences conserved motifs Genetics histidine residue Molecular Biology Gene metal-binding site chemistry.chemical_classification Phylogenetic tree Transporter transmembrane domain Cell Biology Amino acid Transmembrane domain 030104 developmental biology chemistry Biochemistry ZIP transmembrane domain histidine residue metal-binding site conserved motifs General Agricultural and Biological Sciences ZIP 010606 plant biology & botany |
| Zdroj: | Volume: 40, Issue: 3 600-611 Turkish Journal of Biology |
| ISSN: | 1303-6092 1300-0152 |
| Popis: | Filiz, Ertugrul/0000-0001-9636-6389; Ozyigit, Ibrahim Ilker/0000-0002-0825-5951 WOS: 000378689400009 Zinc is an important catalytic element for more than 300 enzymes and plays a structural role in the stabilization of many proteins. Protein domain analysis showed that identified Zn transporter proteins belong to the ZIP protein family (PF02535). Zn transporter sequences were found to have similar molecular weights (33.1-51.4 kDa) and amino acid lengths (306-478 amino acids) with 5.31-8.92 pI values. Subcellular localization of Zn transporters was predicted as the plasma membrane. They had 6-9 putative transmembrane domains with a variable region between TM-3 and TM-4, which could contain a potential histidine-rich metal-binding domain. Moreover, alignment analysis showed that the TM-2, -4, and -5 domains could be potential metal-binding sites because they contain highly conserved His residues. Based on a homology search, the retrieved sequences were identified as corresponding homologs of either Arabidopsis thaliana or Oryza sativa. Phylogenetic analysis also supported that A. thaliana and O. sativa sequences could be used as a reference/benchmark to identify Zn transporter homologs in various plant species. The findings of this study will be valuable theoretical knowledge for feature studies in terms of understanding the gene and protein features of Zn transporters in various plants. |
| Databáze: | OpenAIRE |
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