Chromosomal mapping of repetitive sequences in Hyphessobrycon eques (Characiformes, Characidae): a special case of the spreading of 5S rDNA clusters in a genome
| Autor: | Lucas Baumgärtner, Leonardo Marcel Paiz, Fiorindo José Cerqueira, Patricia Pasquali Parise-Maltempi, Vladimir Pavan Margarido, Carlos Alexandre Fernandes, Diovani Piscor, Roberto Laridondo Lui |
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| Přispěvatelé: | Universidade Estadual do Oeste do Paraná (UNIOESTE), Universidade Estadual de Mato Grosso do Sul (UEMS), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2020 |
| Předmět: |
Male
0106 biological sciences 0301 basic medicine Hyphessobrycon eques Retrotransposon Plant Science Biology DNA Ribosomal 010603 evolutionary biology 01 natural sciences Genome Chromosomes 03 medical and health sciences Species Specificity Centromere RNA Ribosomal 18S Genetics Animals Microsatellites Repeated sequence In Situ Hybridization Fluorescence Repetitive Sequences Nucleic Acid 5S-Sauria 18S rDNA Characidae RNA Ribosomal 5S Chromosome Mapping Chromosome General Medicine biology.organism_classification U2 snDNA Diploidy H3 histone 030104 developmental biology Karyotyping Insect Science Chromosomal region Microsatellite Female Animal Science and Zoology Characiformes Microsatellite Repeats |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1573-6857 0016-6707 |
| DOI: | 10.1007/s10709-020-00086-3 |
| Popis: | Made available in DSpace on 2020-12-12T01:13:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-02-01 Cytogenetic data showed a variation in diploid chromosome number in the genus Hyphessobrycon ranging from 2n = 46 to 52, and studies involving repetitive DNA sequences are scarce in representatives of this genus. The purpose of this paper was the chromosomal mapping of repetitive sequences (rDNA, histone genes, U snDNA and microsatellites) and investigation of the amplification of 5S rDNA clusters in the Hyphessobrycon eques genome. Two H. eques populations displayed 2n = 52 chromosomes, with the acrocentric pair No. 24 bearing Ag-NORs corresponding with CMA3+/DAPI−. FISH with a 18S rDNA probe identified the NORs on the short (p) arms of the acrocentric pairs Nos. 22 and 24. The 5S rDNA probe visualized signals on almost all chromosomes in genomes of individuals from both populations (40 signals); FISH with H3 histone probe identified two chromosome pairs, with the pericentromeric location of signals; FISH with a U2 snDNA probe identified one chromosome pair bearing signals, on the interstitial chromosomal region. The mononucleotide (A), dinucleotide (CA) and tetranucleotide (GATA) repeats were observed on the centromeric/pericentromeric and/or terminal positions of all chromosomes, while the trinucleotide (CAG) repeat showed signals on few chromosomes. Molecular analysis of 5S rDNA and non-transcribed spacers (NTS) showed microsatellites (GATA and A repeats) and a fragment of retrotransposon (SINE3/5S-Sauria) inside the sequences. This study expanded the available cytogenetic data for H. eques and demonstrated to the dispersion of the 5S rDNA sequences on almost all chromosomes. Centro de Ciências Biológicas e da Saúde Laboratório de Citogenética Universidade Estadual do Oeste do Paraná (UNIOESTE), Rua Universitária, 2069 Universidade Estadual de Mato Grosso do Sul (UEMS) Unidade de Mundo Novo, BR 163, Km 20.2 Instituto de Biociências Departamento de Biologia Laboratório de Citogenética Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Av. 24A, 1515 Instituto de Biociências Departamento de Biologia Laboratório de Citogenética Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Av. 24A, 1515 |
| Databáze: | OpenAIRE |
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