Human immunodeficiency virus type 1 envelope glycoprotein gp120 produces immune defects in CD4+ T lymphocytes by inhibiting interleukin 2 mRNA
Autor: | W W Hall, Vaniambadi S. Kalyanaraman, Narendra Chirmule, M Shuster, Naoki Oyaizu, Savita Pahwa, R Pahwa |
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Rok vydání: | 1990 |
Předmět: |
Interleukin 2
T-Lymphocytes medicine.medical_treatment Clone (cell biology) HIV Envelope Protein gp120 Lymphocyte Activation chemistry.chemical_compound Immune system Antigens CD medicine Humans RNA Messenger Cells Cultured Multidisciplinary biology Cell Membrane Immunosuppression Blotting Northern Envelope glycoprotein GP120 Molecular biology Clone Cells chemistry CD4 Antigens Ionomycin HIV-1 Phorbol biology.protein Interleukin-2 Signal transduction Research Article medicine.drug |
Zdroj: | Proceedings of the National Academy of Sciences. 87:2379-2383 |
ISSN: | 1091-6490 0027-8424 |
Popis: | Envelope glycoprotein gp120 of human immunodeficiency virus type 1 (HIV-1) is known to inhibit T-cell function, but little is known about the mechanisms of this immunosuppression. Pretreatment of a CD4+ tetanus toxoid-specific T-cell clone with soluble gp120 was found to exert a dose-dependent inhibition of soluble antigen-driven or anti-CD3 monoclonal antibody-driven proliferative response, interleukin 2 (IL-2) production, and surface IL-2 receptor (IL-2R) alpha-chain expression, all of which were reversed by the addition of exogenous IL-2. mRNA for the gene encoding IL-2 was suppressed by treatment with gp120, but IL-2R gene transcription was not inhibited. Bypass activation of the T-cell clone with phorbol 12-myristate 13-acetate plus ionomycin was unaffected by gp120 pretreatment. Thus, gp120-CD4 interaction interferes with an essential role of the CD4 molecule in signal transduction through the CD3-antigen receptor (Ti) complex. Such a mechanism of gp120-induced immunosuppression, if operative in vivo, could contribute to the depressed specific immune responses associated with HIV infection. |
Databáze: | OpenAIRE |
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