Comparative RNA-Seq analysis reveals genes associated with masculinization in female Cannabis sativa
| Autor: | Ayelign M. Adal, Larry A. Holbrook, Soheil S. Mahmoud, Ketan Doshi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Stamen RNA-Seq Flowers Plant Science Cannabis sativa medicine.disease_cause Flower sex 01 natural sciences 03 medical and health sciences Differential expression Gene Expression Regulation Plant Pollen Genetics medicine Transcription factor Gene Cannabis Plant Proteins 030304 developmental biology 0303 health sciences biology Gene Expression Profiling fungi food and beverages biology.organism_classification Phenotype Plant Breeding Original Article Male biased-genes Silver thiosulfate 010606 plant biology & botany |
| Zdroj: | Planta |
| ISSN: | 1432-2048 0032-0935 |
| Popis: | Main conclusionUsing RNA profiling, we identified several silver thiosulfate-induced genes that potentially control the masculinization of femaleCannabis sativaplants.AbstractGenetically femaleCannabis sativaplants normally bear female flowers, but can develop male flowers in response to environmental and developmental cues. In an attempt to elucidate the molecular elements responsible for sex expression inC. sativaplants, we developed genetically female lines producing both female and chemically-induced male flowers. Furthermore, we carried out RNA-Seq assays aimed at identifying differentially expressed genes responsible for male flower development in female plants. The results revealed over 10,500 differentially expressed genes, of which around 200 potentially control masculinization of female cannabis plants. These genes include transcription factors and other genes involved in male organ (i.e., anther and pollen) development, as well as genes involved in phytohormone signalling and male-biased phenotypes. The expressions of 15 of these genes were further validated by qPCR assay confirming similar expression patterns to that of RNA-Seq data. These genes would be useful for understanding predisposed plants producing flowers of both sex types in the same plant, and help breeders to regulate the masculinization of female plants through targeted breeding and plant biotechnology. |
| Databáze: | OpenAIRE |
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