Effects of glycerol on VLDL secretion by the isolated rat liver
| Autor: | A. Raisonnier, Recaredo Infante, M.E. Bouma, D. Petit |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Glycerol
Very low-density lipoprotein medicine.medical_specialty Biophysics Biological Transport Active Oleic Acids Stimulation Lipoproteins VLDL Biochemistry chemistry.chemical_compound Endocrinology Leucine Internal medicine medicine Animals chemistry.chemical_classification Chemistry Fatty acid Lipid metabolism Metabolism Rats Perfusion Kinetics Liver Lipogenesis lipids (amino acids peptides and proteins) |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 431:481-492 |
| ISSN: | 0005-2760 |
| Popis: | Stimulation of VLDL production by increasing fatty acid availability is now well established. However, a possible regulatory role of glycerol, another lipid precursor, in VLDL synthesis by the liver has not yet been substantiated. The present experiments investigate this problem using the isolated perfused rat liver. [14C] Glycerol uptake and metabolism were studied at two different glycerol concentrations: 1 μmol/perfusate (control) or 1.6 mmol/perfusate. VLDL production and lipid synthesis were investigated using [14C]leucine and several labelled fatty acids as precursors in control and glycerol-overloaded livers. Neoglycogenesis and lipogenesis from glycerol carbons are negligible in our conditions. The absolute amount of glycerol, but not the percentage, taken up by the liver, increased after raising its concentration in the perfusate. A major part of exogenous (plasmatic) glycerol was esterified with endogenous (non plasmatic) fatty acids. Incorporation of radioactive fatty acids into liver or plasma lipids was lower than in the control group. Significant differences were observed between saturated and unsaturated fatty acids used as lipid precursors. Production of VLDL as assessed by radioactive leucine and fatty acid incorporation in the VLDL of the perfusate was depressed by glycerol. Glycerol partly inhibits the normal stimulation of VLDL production by plasmatic fatty acid overload. |
| Databáze: | OpenAIRE |
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