Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiols
| Autor: | M. F. Giordana, G. Ulloa, Nicolás Órdenes-Aenishanslins, B. Collao, F. A. Venegas, L. A. Saona, J. P. Monrás, Denisse Bravo, José M. Pérez-Donoso |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Green chemistry
Adenosine monophosphate CADMIUM SULPHIDE General Chemical Engineering Otras Ciencias Biológicas SULFUR Nanoparticle Context (language use) Nanotechnology HYDROGEN-SULFIDE 02 engineering and technology 010402 general chemistry 01 natural sciences BIOMIMETIC SYNTHESIS AQUEOUS-SOLUTION Nanomaterials Ciencias Biológicas purl.org/becyt/ford/1 [https] chemistry.chemical_compound NANOPARTICLES SEMICONDUCTOR NANOCRYSTALS BIOSYNTHESIS purl.org/becyt/ford/1.6 [https] technology industry and agriculture IN-VITRO CDTE General Chemistry Glutathione equipment and supplies 021001 nanoscience & nanotechnology Phosphate Combinatorial chemistry 0104 chemical sciences chemistry ESCHERICHIA-COLI Quantum dot BACTERIA 0210 nano-technology CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Rsc Advances Artículos CONICYT CONICYT Chile instacron:CONICYT |
| Popis: | Developing methods with a low environmental impact for nanoparticle synthesis remains one of the greatest challenges in nanotechnology. In this context, biomimetic and biological methods have emerged as green chemistry alternatives, and also contribute to our understanding of how nanomaterials interact with cellular molecules. Here, we report a phosphate-dependent biomimetic method to synthesize of cadmium sulphide (CdS) QDs at low temperatures, physiological pH and aerobic conditions, using CdCl2 and thiols (l-cysteine, glutathione or mercaptosuccinic acid). Inorganic phosphate (Pi) and cellular phosphorylated intermediates such as adenosine monophosphate, glucose-6-phosphate, glycerol-2-phosphate and fructose-1,6-biphosphate, can trigger CdS QDs synthesis. The produced QDs are cubic phase nanocrystals with a tunable fluorescence (450-700 nm), small size (4-12 nm), and are composed of thiols and Pi. In CdS synthesis, the importance of the phosphate is related to its capacity to release H2S from thiols, a phenomenon associated with its base-properties. Based on the biomimetic method, we developed a Pi-based procedure to synthesize CdS QDs in Escherichia coli. As in the biomimetic procedure, Pi favors QDs-biosynthesis not only by mediating biological generation of H2S, but also by improving Cd2+ cellular uptake. A role for phosphates in the cellular interaction and green synthesis of metal QDs is discussed. Fil: Venegas, F. A.. Universidad Andrés Bello; Chile Fil: Saona, L. A.. Universidad Andrés Bello; Chile. Universidad de Chile; Chile Fil: Monrás, J. P.. Universidad Andrés Bello; Chile Fil: Órdenes Aenishanslins, N.. Universidad Andrés Bello; Chile. Universidad de Chile; Chile Fil: Giordana, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina Fil: Ulloa, G.. Universidad Andrés Bello; Chile. Universidad de Chile; Chile Fil: Collao, B.. Universidad Andrés Bello; Chile Fil: Bravo, D.. Universidad de Chile; Chile Fil: Pérez Donoso, José M.. Universidad Andrés Bello; Chile |
| Databáze: | OpenAIRE |
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