Novel natural genetic variation controlling the competence to form adventitious roots and shoots from the tomato wild relative Solanum pennellii
| Autor: | Danielle Camargo Scotton, Maísa de Siqueira Pinto, Antonio Figueira, Fernanda Namie Arikita, Lázaro Eustáquio Pereira Peres, Mariana da Silva Azevedo |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
food.ingredient
DNA Plant Genotype Introgression Plant Science Breeding Solanum Models Biological Plant Roots Chromosomes Plant Murashige and Skoog medium food Genetic variation Botany Genetics Regeneration Gene biology Genetic Variation General Medicine biology.organism_classification Seedlings Mutation Shoot Cotyledon Agronomy and Crop Science Plant Shoots Explant culture |
| Zdroj: | Plant Science. :121-130 |
| ISSN: | 0168-9452 |
| Popis: | a b s t r a c t Tomato (Solanum lycopersicum L.) is an attractive model to study the genetic basis of adventitious organ formation capacity, since there is considerable natural genetic variation among wild relatives. Using a set of 46 introgression lines (ILs), each containing a small chromosomal segment of Solanum pennellii LA716 introgressed and mapped into the tomato cultivar M82, we characterized a high shoot-regeneration capacity for ILs 3-2, 6-1, 7-1, 7-2, 8-2, 8-3, 9-1, 9-2, 10-2 and 10-3, when cotyledon explants were culti- vated on medium containing 5.0 M BAP. F1 seedlings from the crosses 'Micro-Tom × ILs' and 'ILs × ILs' demonstrated that the shoot regeneration capacity of most ILs was dominant and that the regeneration ability of IL8-3 enhanced that of the other ILs in an additive manner. The ILs 3-2, 7-1, 8-3, and 10-2 also exhibited enhanced root formation on MS medium containing 0.4 M NAA, indicating that these chro- mosomal segments may contain genes controlling the competence to assume distinct cell fates, rather than the induction of a specific organ. We also performed the introgression of the genes controlling competence into the model system 'Micro-Tom'. The further isolation of such genes will improve our understanding of the molecular basis of organogenic capacity. © 2012 Elsevier Ireland Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect