VIP plasma levels associate with survival in severe COVID-19 patients, correlating with protective effects in SARS-CoV-2-infected cells
| Autor: | Caroline S. de Freitas, Lívia Teixeira, Suelen da Silva Gomes Dias, Jairo R. Temerozo, Eugenio D. Hottz, Carolina Q. Sacramento, Isaclaudia G. de Azevedo-Quintanilha, André C. Ferreira, Dumith Chequer Bou-Habib, Thiago Moreno L. Souza, Pedro Kurtz, Mayara Mattos, Fernando A. Bozza, Vinicius Cardoso Soares, Patrícia T. Bozza, Camila R. R. Pão, Natalia Fintelman-Rodrigues |
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| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
Programmed cell death Receptors Vasoactive Intestinal Polypeptide Type I Immunology Neuropeptide CREB Proinflammatory cytokine Immunology and Allergy Humans Medicine Transcription factor biology SARS-CoV-2 business.industry NF-kappa B COVID-19 Lipid metabolism Cell Biology In vitro Viral replication biology.protein Pituitary Adenylate Cyclase-Activating Polypeptide RNA Viral business hormones hormone substitutes and hormone antagonists Transcription Factors Vasoactive Intestinal Peptide |
| DOI: | 10.1101/2020.07.25.220806 |
| Popis: | Infection by SARS-CoV-2 may elicit uncontrolled and damaging inflammatory responses. Thus, it is critical to identify compounds able to inhibit virus replication and thwart the inflammatory reaction. Here, we show that the plasma levels of the immunoregulatory neuropeptide VIP are elevated in patients with severe COVID-19, correlating with reduced inflammatory mediators and with survival on those patients. In vitro, vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP), highly similar neuropeptides, decreased the SARS-CoV-2 RNA content in human monocytes and viral production in lung epithelial cells, also reducing cell death. Both neuropeptides inhibited the production of proinflammatory mediators in lung epithelial cells and in monocytes. VIP and PACAP prevented in monocytes the SARS-CoV-2-induced activation of NF-kB and SREBP1 and SREBP2, transcriptions factors involved in proinflammatory reactions and lipid metabolism, respectively. They also promoted CREB activation, a transcription factor with antiapoptotic activity and negative regulator of NF-kB. Specific inhibition of NF-kB and SREBP1/2 reproduced the anti-inflammatory, antiviral, and cell death protection effects of VIP and PACAP. Our results support further clinical investigations of these neuropeptides against COVID-19. |
| Databáze: | OpenAIRE |
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