T cells stimulated in vitro have a suppressive function but do not contain only regulatory T cells
| Autor: | Marilena Granzotto, Alberto Tommasini, Elisa Piscianz, Erica Valencic |
|---|---|
| Přispěvatelé: | Valencic, E., Piscianz, E., Tommasini, A., Granzotto, M. |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cells T-Lymphocytes Immunology chemical and pharmacologic phenomena Biology Lymphocyte Activation T-Lymphocytes Regulatory Immunophenotyping Interleukin 21 Interferon-gamma Basic Immunology CD4-Positive T-Lymphocytes Cell Proliferation Cells Cultured Forkhead Transcription Factors Humans Immune Tolerance Immunophenotyping Interferon-gamma Interleukin-2 Interleukin-2 Receptor alpha Subunit Lymphocyte Activation Phytohemagglutinins T-Lymphocytes Regulatory Immune Tolerance Immunology and Allergy Cytotoxic T cell Humans IL-2 receptor Phytohemagglutinins Antigen-presenting cell Cells Cultured Cultured Forkhead Transcription Factors Humans Immune Tolerance Immunophenotyping Interferon-gamma Interleukin-2 Interleukin-2 Receptor alpha Subunit Lymphocyte Activation Phytohemagglutinins T-Lymphocyte Interleukin 3 Cell Proliferation Cultured Interleukin-2 Receptor alpha Subunit FOXP3 hemic and immune systems Forkhead Transcription Factors Natural killer T cell CD4-Positive T-Lymphocytes Cell Proliferation Cell Interleukin 12 Interleukin-2 |
| Popis: | Summary The generation of regulatory T cells (Tregs) in vitro represents an attractive possibility to set up cellular therapies that could prevent and cure autoimmune disorders. Different methods have been proposed to generate Tregs in vitro and to evaluate their phenotype and function. Moreover, the overlap between generation of activated and regulatory cells could often be underestimated. We showed that in vitro treatment of CD4+ CD25– lymphocytes with different stimuli leads to a good expression of CD25 and forkhead box P3 (FoxP3) on most cells, but to a full Treg phenotype (including CD127 negativity) in only a minor percentage of cells, ranging from 17·38% of cells treated with phytohaemagglutinin (PHA) to 50·91% of cells treated with T cell receptor (TCR) stimulation in association with transforming growth factor (TGF)-β. Some suppressive activity was demonstrated for T cells activated with all the different stimuli. However, while suppression mediated by TCR/TGF-β treated T cells was associated with an inhibition of both interleukin (IL)-2 and interferon (IFN)-γ in the co-culture supernatant, the suppression observed for PHA-activated cells occurred in the presence of large amounts of these cytokines. In conclusion, also taking into account other recent publications, caution should be taken in interpretation of data in the field of regulatory T cells. |
| Databáze: | OpenAIRE |
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