Fluorescent Determination of Calcium Ion Using a Coumarinyl Pyrazoline Scaffold and Its Application in Living Cells
| Autor: | Yun Hua, Ya-jing Shang, Gang Wu, Qiang Wei, Zhi-Bin Sun |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Scaffold
010401 analytical chemistry Biochemistry (medical) Clinical Biochemistry chemistry.chemical_element Pyrazoline 02 engineering and technology Calcium 021001 nanoscience & nanotechnology Coumarin 01 natural sciences Biochemistry Fluorescence 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY Electrochemistry Biophysics heterocyclic compounds 0210 nano-technology Spectroscopy Intracellular |
| DOI: | 10.6084/m9.figshare.10290641.v1 |
| Popis: | Calcium is an essential element for living systems and involved in numerous intracellular signaling cascades. In this work, a novel fluorescent probe based on a coumarin and pyrazoline scaffold was designed and synthesized for the detection of Ca2+ by a photoinduced electron transfer (PET) strategy. The probe showed good selectivity for Ca2+ compared to other substances in ethanol with a low detection limit (1.37 × 10−7 M). Meanwhile, following the addition of Ca2+ ions, the probe showed an obvious “turn on” with a rapid response of less than 5 s and 1:1 binding with an association constant of 7.97 × 103 M−1. Furthermore, the fluorescence imaging of the probe was also performed in the presence of Ca2+ in living A549 cells. |
| Databáze: | OpenAIRE |
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