Genetic Polymorphisms in DEFB1 and miRNA202 Are Involved in Salivary Human β-Defensin 1 Levels and Caries Experience in Children
| Autor: | Daniela Silva Barroso de Oliveira, Leonardo Santos Antunes, Andrea Lips, Raquel Assed Bezerra da Silva, Léa Assed Bezerra da Silva, Erika Calvano Küchler, Lívia Azeredo Alves Antunes, Paulo Nelson-Filho, Júlia Guimarães Barcellos de Abreu, Driely Barreiros, Gutemberg Gomes Alves, Ana Carolina Batista |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Saliva beta-Defensins Genotype Dental Caries Susceptibility Enzyme-Linked Immunosorbent Assay Dental Caries Real-Time Polymerase Chain Reaction Polymorphism Single Nucleotide CRIANÇAS 03 medical and health sciences 0302 clinical medicine Saliva collection Humans Medicine Statistical analysis General Dentistry Defensin Alleles Genetics Caries susceptibility business.industry 030206 dentistry MicroRNAs 030104 developmental biology Child Preschool Dental examination Immunology Female business Caries experience Brazil |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1421-976X 0008-6568 |
| Popis: | The antimicrobial peptides human β-defensins (hBDs) are encoded by β-defensin genes (DEFBs) and are possibly involved in caries susceptibility. In this study we aimed (1) to investigate the relationship between salivary hBDs and caries and (2) to evaluate the association of genetic polymorphisms in DEFB1 and microRNA202 (miRNA202) with salivary levels of hBDs and caries experience. Two data sets were available for this study, totalizing 678 Brazilian children. Dental examination and saliva collection were performed in all included children. The salivary level for hDB1, hBD2, and hBD4 was assessed by ELISA sandwich technique in 168 children. The DNA was extracted from saliva, and polymorphisms in DEFB1 and miRNA202 were analyzed by real-time PCR. Statistical analysis was performed to investigate the associations between caries experience, hBD salivary level, genotype, and allele distribution, with an alpha of 0.05. The hBD1 level was significantly higher in caries-free children (p < 0.0001). The miRNA202 was associated with a lower level of salivary hBD1 (p < 0.05). Also, the polymorphic distribution of miRNA202 was associated with caries (p = 0.006). The polymorphisms in DEFB1 were not associated with hBD salivary level and caries experience (p > 0.05). In conclusion, our results indicate that genetic polymorphism in miRNA202 is involved in hBD1 salivary level as well as caries experience in children. |
| Databáze: | OpenAIRE |
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