Molecular identification and phylogenetic analysis of Bothrops insularis bacterial and fungal microbiota
| Autor: | Eduardo Bagagli, Tarsila Frachin, Jéssica Luana Chechi, Ary Fernandes Júnior, Priscila Luiza Mello, Hans Garcia Garces, Benedito Barraviera, Luciana Curtolo de Barros, Sandra De Moraes Guimenes Bosco, Lucilene Delazari dos Santos, Rui Seabra Ferreira, Lidiane Nunes Barbosa |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
DNA Bacterial Male Bothrops insularis 040301 veterinary sciences Aerobic bacteria Health Toxicology and Mutagenesis Zoology Fungus Biology Toxicology Enterococcus faecalis 0403 veterinary science 03 medical and health sciences Phylogenetics parasitic diseases Animals Bothrops DNA Fungal Phylogeny Phylogenetic tree Bacteria Fungi 04 agricultural and veterinary sciences 16S ribosomal RNA biology.organism_classification 030104 developmental biology GenBank Female Brazil Mycobiome |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1528-7394 |
| Popis: | Made available in DSpace on 2018-12-11T17:17:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-03-19 Bothrops insularis, known as the golden lancehead snake, has its natural habitat restricted to Queimada Grande Island on the southern coast of Brazil. This culture-dependent study aimed to identify microorganisms obtained from the mouth, eyes, and cloaca of this species. Swabs from 20 snakes were collected for fungal and bacterial isolation. DNA was extracted from all samples, and identification was performed by amplifying the ITS1-5.8S-ITS2 regions and the 16S rDNA gene, respectively. All strains were identified and deposited in the GenBank nucleotide database. MEGA v6.0 software was utilized to construct phylogenetic trees. In total, 100 strains were isolated and characterized, from which 42 fungi were distributed into 23 species and 58 bacteria into 13 species. The genus Fusarium was predominant since 11 strains and probably a new species was isolated from this fungus. Pseudomonas aeruginosa and Enterococcus faecalis were the predominant groups of aerobic bacteria isolated. Phylogenetic analyses between bacterial and fungal sequences suggest a similarity between the microorganisms found on the island and on the continent. These findings may be attributed to anthropic actions resulting from both expeditions to the island and actions of migratory birds, which are the main sources of food for snakes. Center for the Study of Venoms and Venomous Animals (CEVAP) Sao Paulo State University (UNESP) Department of Microbiology and Immunology Institute of Biosciences of Botucatu Sao Paulo State University (UNESP) Graduate Program in Tropical Diseases Botucatu Medical School Sao Paulo State University (UNESP) Center for the Study of Venoms and Venomous Animals (CEVAP) Sao Paulo State University (UNESP) Department of Microbiology and Immunology Institute of Biosciences of Botucatu Sao Paulo State University (UNESP) Graduate Program in Tropical Diseases Botucatu Medical School Sao Paulo State University (UNESP) |
| Databáze: | OpenAIRE |
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