Loop-Mediated Isothermal Amplification (LAMP) Assay to De-tect Toxoplasmosis in Schizophrenia Patients
| Autor: | Shirzad Fallahi, Raheleh Hasanzadeh, Alireza Badirzadeh, Mahdi Khoshsima Shahraki, Hadi Mirahmadi, Hamid Malek Raeesi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Loop-mediated isothermal amplification (LAMP) 030231 tropical medicine 030106 microbiology Loop-mediated isothermal amplification Serology lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine Internal medicine Medicine Seroprevalence Psychiatric hospital lcsh:RC109-216 biology business.industry toxoplasma gondii Zoonosis loop-mediated isother-mal amplification (lamp) Toxoplasma gondii biology.organism_classification medicine.disease Toxoplasmosis schizophrenia Infectious Diseases Schizophrenia Parasitology Original Article business toxoplasmosis |
| Zdroj: | Iranian Journal of Parasitology, Vol 15, Iss 3, Pp 299-306 (2020) Iranian Journal of Parasitology |
| ISSN: | 1735-7020 |
| Popis: | Background: Toxoplasma gondii (T. gondii) causes an important parasitic infection known as toxoplasmosis, which is a globally distributed important zoonosis. One of the major serious characteristics of T. gondii is its ability to manipulate the behavior of intermediate hosts. We performed a cross-sectional study to determine toxoplasmosis in schizophrenic patients, as one of the major neuropsychiatric disorders, using loop-mediated isothermal amplification (LAMP) technic by targeting parasite B1 gene. Methods: Blood samples were taken from 118 schizophrenic patients hospitalized in tow hospitals including Baharan, Clinic of Psychiatric Ali-ibn-Abi-Talib Hospital (in Zahedan City), and Amir-al Momenin Psychiatric Hospital (in Zabol City), Sistan and Baluchestan Province, southeast Iran in 2016. They were analyzed using LAMP, and compared with the previous data of nested-PCR and serology. Results: Out of the 118 schizophrenic individuals, 56 patients (47.4%) were found to be infected with T. gondii. The diagnosis of toxoplasmosis was confirmed in 41 patients (34.7%) via the nested-PCR. The seroprevalence of toxoplasmosis in schizophrenic patients was 55.9% (66/118). Conclusion: We found a high efficiency of LAMP method in identifying toxoplasmosis and its high prevalence among schizophrenic patients. Our findings could provide viable offer implications for the prevention of schizophrenia. |
| Databáze: | OpenAIRE |
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