Synergistic stimulation of alkaline phosphatase activity in bovine aortic endothelial cells grown in the presence of retinoids and glucocorticoids
Autor: | Susan E. Adams, George Melnykovych |
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Rok vydání: | 1985 |
Předmět: |
medicine.medical_specialty
Retinyl Esters Physiology medicine.medical_treatment Clinical Biochemistry Retinoic acid Tretinoin Biology Retinyl acetate Triamcinolone Acetonide Dexamethasone chemistry.chemical_compound Retinoids Internal medicine medicine Animals Endothelium Vitamin A Glucocorticoids Aorta Dose-Response Relationship Drug Retinol Drug Synergism Cell Biology Alkaline Phosphatase Elevated alkaline phosphatase Steroid hormone Endocrinology chemistry Alkaline phosphatase Cattle medicine.symptom Diterpenes Glucocorticoid medicine.drug |
Zdroj: | Journal of cellular physiology. 124(1) |
ISSN: | 0021-9541 |
Popis: | Low concentrations of retinol (10 nM-10 microM) and dexamethasone (0.1 nM-1.0 microM) elevated activity of alkaline phosphatase (E.C. 3.1.3.1) in bovine endothelial cells in culture. The effect was apparent after 48 hr of growth in the presence of either of the two compounds, prior to any growth stimulatory effects. A synergistic stimulation of alkaline phosphatase was achieved in the presence of both retinol and dexamethasone implying different mechanisms of induction. Retinoic acid and retinyl acetate also elevated alkaline phosphatase but the retinol analogue reduced in the side chain (perhydroretinol) was inactive. The ability of steroids to elevate alkaline phosphatase activity was associated with the structure commonly required for glucocorticoid activity; however, competitors for the steroid receptor binding failed to prevent the elevation by dexamethasone or the synergism in the presence of retinol and dexamethasone. |
Databáze: | OpenAIRE |
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