W246G Mutant ELOVL4 Impairs Synaptic Plasticity in Parallel and Climbing Fibers and Causes Motor Defects in a Rat Model of SCA34
| Autor: | Richard S. Brush, Ferenc Deak, David M. Sherry, Albert Orock, Jennifer L. Fessler, Raghavendra Y. Nagaraja, Robert E. Anderson, Karanpreet Multani, Megan Stiles, Feng Li, Martin-Paul Agbaga, Mohiuddin Ahmad |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Cerebellum Ataxia Motor Disorders Neuroscience (miscellaneous) Parallel fiber Biology Nerve Fibers Myelinated Article 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Organ Culture Techniques medicine Animals Spinocerebellar Ataxias Rats Long-Evans Spinocerebellar ataxia-34 (SCA34) Eye Proteins Very Long Chain Fatty Acids (VLC-FA) Neuronal Plasticity Membrane Proteins Long-term potentiation Elongation of Very Long Chain Fatty Acids-4 (ELOVL4) Climbing fiber medicine.disease Rats Electrophysiology 030104 developmental biology medicine.anatomical_structure Neurology Synaptic plasticity Mutation Spinocerebellar ataxia Cerebellar atrophy Female medicine.symptom Rats Transgenic Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Molecular Neurobiology |
| ISSN: | 1559-1182 0893-7648 |
| Popis: | Background: Spinocerebellar ataxias (SCA) are a group of neurodegenerative disorders characterized by neuronal degeneration leading to loss of motor coordination. A number of different mutations gives rise to different types of SCA with characteristic ages of onset, symptomatology, and rates of progression. SCA type 34 (SCA34) is an age-related cerebellar neurodegenerative disorder caused by mutations in the fatty acid elongase-4 (ELOVL4). The ELOVL4 is an essential enzyme that mediates biosynthesis of Very Long Chain Saturated and Polyunsaturated Fatty Acids (VLC-SFA and VLC-PUFA, resp., ≥28 carbons) that are critical for the normal function of brain, skin, retina, Meibomian glands, and testes in which ELOVL4 is expressed. Global deletion or homozygous expression of truncated mutant ELOVL4 that lack VLC-SFA and VLC-PUFA biosynthesis cause severe skin disorders, seizures and neonatal mortality in rodents and humans. Methods: To understand role of ELOVL4 and its products in neuronal function and to evaluate the consequences of ELOVL4 mutations in pathogenesis of age-related SCA34, we generated a rat model of SCA34 by knock-in of the SCA34-causing 736T>G (p.W246G) ELOVL4 mutation. We performed biochemical, neuroanatomical and behavioral analyses by rotorod to measure motor function. We used electrophysiological recordings from cerebellar slices to determine the impact of the W246G mutation on neuronal function. Results were analyzed using GraphPad Prism Statistical software. Results: Heterozygous and homozygous rats carrying the W246G mutation developed impaired motor deficits by two months of age. To understand the mechanism of these motor deficits, we performed electrophysiological studies using cerebellar slices from rats homozygous for W246G mutant ELOVL4 and found marked reduction of long-term potentiation at parallel fiber synapses and long-term depression at climbing fiber synapses onto Purkinje cells. Neuroanatomical analysis of the cerebellum up 6 months of age showed normal cytoarchitectural organization despite the early-onset motor deficits and defects in synaptic plasticity. Conclusions: Our results point to ELOVL4 and its products being essential for motor function and cerebellar synaptic plasticity. The results further suggest that in SCA34 patients, ataxia arises from primary impairment of synaptic plasticity and cerebellar network desynchronization that precedes cerebellar degeneration and loss of motor coordination with aging. |
| Databáze: | OpenAIRE |
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