Comparative Study of Human and Automated Screening for Antinuclear Antibodies by Immunofluorescence on HEp-2 Cells
| Autor: | Marco Cipolla, Raja Marrakchi Triki, Giuseppe Friscia, Vincenzo Taormina, Donato Cascio, Hechmi Louzir, Alessandro Fauci, Giuseppe Raso, Imen Sfar, Asma Gati, Ignazio Brusca, Taieb Ben Abdallah, Youssra Haouami, Melika Ben Ahmed, Tarak Dhaouadi, Yousr Gorgi, Amel Benammar Elgaaied |
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| Přispěvatelé: | Gorgi Y, Dhaouadi T, Sfar I, Haouami Y, Ben Abdallah T, Raso G, Cascio D, Cipolla M, Taormina V, Fauci A, Brusca I, Friscia G, Benammar Elgaaïed A, Marrakchi Triki R, Gati A, Ben Ahmed M, Louzir H |
| Rok vydání: | 2015 |
| Předmět: |
Statistics and Probability
Pathology medicine.medical_specialty Indirect immunofluorescence Anti-nuclear antibody medicine.diagnostic_test business.industry Concordance Health Informatics IIf Immunofluorescence Health Professions (miscellaneous) Settore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin) Continuous integration Pattern identification Autoantibodies HEp-2 Immunofluorescence autoimmune diseases automated screening standardization Health Information Management Medicine Fine speckled business |
| Zdroj: | International Journal of Statistics in Medical Research. 4:270-276 |
| ISSN: | 1929-6029 |
| Popis: | Background : Several automated systems had been developed in order to reduce inter-observer variability in indirect immunofluorescence (IIF) interpretation. We aimed to evaluate the performance of a processing system in antinuclear antibodies (ANA) screening on HEp-2 cells. Patients and Methods : This study included 64 ANA-positive sera and 107 ANA-negative sera that underwent IIF on two commercial kits of HEp-2 cells (BioSystems® and Euroimmun®). IIF results were compared with a novel automated interpretation system, the “ Cyclopus CADImmuno®” (CAD). Results : All ANA-positive sera images were recognized as positive by CAD (sensitivity = 100%), while 17 (15.9%) of the ANA-negative sera images were interpreted as positive (specificity = 84.1%), κ=0.799 (SD=0.045). Comparison of IIF pattern determination between human and CAD system revealed on HEp-2 (BioSystems®), a complete concordance in 6 (9.37%) sera, a partial concordance (sharing of at least 1 pattern) in 42 (65.6%) cases and in 16 (25%) sera the pattern interpretation was discordant. Similarly, on HEp-2 (Euroimmun®) the concordance in pattern interpretation was total in 5 (7.8%) sera, partial in 39 (60.9%) and absent in 20 (31.25%). For both tested HEp-2 cells kits agreement was enhanced for the most common patterns, homogenous, fine speckled and coarse speckled. While there was an issue in identification of nucleolar, dots and nuclear membranous patterns by CAD. Conclusion : Assessment of ANA by IIF on HEp-2 cells using the automated interpretation system, the “ Cyclopus CADImmuno®” is a reliable method for positive/negative differentiation. Continuous integration of IIF images would improve the pattern identification by the CAD. |
| Databáze: | OpenAIRE |
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