Effects of caffeine on the structure and conformation of DNA: A force spectroscopy study
| Autor: | Marcio S. Rocha, T.A. Moura, Leandro Licursi de Oliveira |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Optical Tweezers
Ionic bonding Cooperativity Optical tweezers 02 engineering and technology Nucleic Acid Denaturation Biochemistry 03 medical and health sciences Nucleic acid thermodynamics chemistry.chemical_compound Structural Biology Caffeine Molecule Molecular Biology 030304 developmental biology 0303 health sciences Molecular Structure Chemistry Single molecule force spectroscopy Spectrum Analysis Force spectroscopy General Medicine DNA Models Theoretical 021001 nanoscience & nanotechnology Biophysics Nucleic Acid Conformation 0210 nano-technology Algorithms |
| Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| Popis: | Here, we use single molecule force spectroscopy performed with optical tweezers in order to investigate the interaction between Caffeine and the DNA molecule for various different concentrations of the alkaloid and under two distinct ionic strengths of the surrounding buffer. We were able to determine the mechanical changes induced on the double-helix structure due to Caffeine binding, the binding mode and the binding parameters of the interaction. The results obtained show that Caffeine binds to DNA by outside the double-helix with a higher affinity at lower ionic strengths. On the other hand, a considerable cooperativity was found only for sufficient high ionic strengths, suggesting that Caffeine may binding forming dimers and/or trimers along the double-helix under this condition. Finally, it was also shown that Caffeine stabilizes the DNA double-helix upon binding, preventing force-induced DNA melting. |
| Databáze: | OpenAIRE |
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