Significance of appropriate techniques and media for isolation and identification of Ureaplasma urealyticum from clinical specimens
| Autor: | A Parreno, Ruth B. Kundsin, Sharon A. Poulin |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Male
Microbiology (medical) food.ingredient Urogenital System Cervix Uteri Urine medicine.disease_cause Ureaplasma Microbiology Agar plate Mycoplasma food Species Specificity Urethra medicine Humans Agar Cervix Bacteriological Techniques biology Isolation (microbiology) biology.organism_classification Culture Media medicine.anatomical_structure Female Research Article Ureaplasma urealyticum |
| Zdroj: | Journal of Clinical Microbiology. 8:445-453 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.8.4.445-453.1978 |
| Popis: | Controversy over the association of Ureaplasma urealyticum with reproductive failure may be due to methods used to isolate the microorganism. U. urealyticum isolations from clinical material should be done simultaneously in broth and on Shepard's differential agar medium (A7) containing manganese sulfate. Urine sediments result in a 9% (P = 0.0002) higher rate of isolation than than cervical and urethral swabs. Primary isolations may not display standard textbook morphology. Isolated colonies may be present, but brown streaks in cervical mucus or a coalescent haze around epithelial cells in urine sediment may also be seen in areas of concentrated growth. The broth and agar media used, method of incubation, type of specimen, and method of storing specimens before culture are all factors which influence the recovery of U. urealyticum. |
| Databáze: | OpenAIRE |
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