Gamma-ray irradiation modulates PGRMC1 expression and the number of CD56+ and FoxP3+ cells in the tumor microenvironment of endometrial endometrioid adenocarcinoma
| Autor: | Jale Yuzugulen, Dmitry Aleksandrovich Zinovkin, Yulia Anatolievna Lyzikova, Eldar A. Nadyrov, Daniil Rudolfovich Petrenyov, Zahidul Islam Pranjol |
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| Rok vydání: | 2021 |
| Předmět: |
PGRMC1
Tumor microenvironment Endometrial Endometrioid Adenocarcinoma business.industry Gamma rays Gamma ray Gamma ray irradiation FOXP3 chemical and pharmacologic phenomena Biology Contribution Endometrioid endometrial carcinoma Oncology FoxP3 Cancer research Medicine Original Article Radiology Nuclear Medicine and imaging CD56 business |
| Zdroj: | Radiation Oncology Journal |
| ISSN: | 2234-3156 2234-1900 |
| DOI: | 10.3857/roj.2021.00472 |
| Popis: | Purpose\ud Although the conventional gamma ray brachytherapy has been successful in treating endometrioid endometrial adenocarcinoma (EC), the molecular and cellular mechanisms of this anti-tumorigenic response remain unclear. Therefore, we investigated whether gamma ray irradiation induces changes in the number of FoxP3+ T-regulatory lymphocytes (Tregs), CD56+ natural killer cells (NK), and the expression of progesterone receptor membrane component 1 (PGRMC1) in the tumor microenvironment (TME).\ud \ud Materials and Methods\ud According to the inclusion criteria, 127 cases were selected and grouped into irradiation-treated (Rad+) and control (underwent surgery) groups and analyzed using immunohistochemistry. Predictive prognostic values were analyzed using Mann-Whitney U test, ROC analysis, relative risk, log-rank, Spearman rank tests and multivariate Cox’s regression.\ud \ud Results\ud We observed significant differences (p < 0.001) between the radiation-treated patients and the control groups in FoxP3+ Tregs numbers, CD56+ NK cells and PGRMC1 expression. Gamma ray induced a 3.71- and 3.39-fold increase in the infiltration of FoxP3+ cells, CD56+ NK cells, respectively and 0.0034-fold change in PGRMC1 expression. Univariate and multivariate analyses revealed predictive role of the parameters. In the irradiated patients’ group, inverted correlations between clinical unfavorable outcome, FoxP3+ Tregs and CD56+ NK cells were observed.\ud \ud Conclusion\ud Our results suggest an immune-modulating role, specifically by increasing immune cell infiltration, of gamma radiation in the TME which may potentially be utilized as biomarkers in prognostic values. |
| Databáze: | OpenAIRE |
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