Genes Involved in Yellow Pigmentation of Cronobacter sakazakii ES5 and Influence of Pigmentation on Persistence and Growth under Environmental Stress▿
| Autor: | Angelika Lehner, Isabel Hartmann, Sophia Johler, Roger Stephan, Kirsten A. Kuehner |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Operon Ultraviolet Rays Mutant Mutagenesis (molecular biology technique) Environment Applied Microbiology and Biotechnology Radiation Tolerance Microbiology Cell Line Mice Cronobacter sakazakii Osmotic Pressure Stress Physiological Animals Humans Cronobacter Gene DNA Primers Genetics Ecology biology Base Sequence Pigmentation Macrophages Wild type Enterobacteriaceae Infections Infant Newborn Pigments Biological biology.organism_classification Carotenoids Infant Formula Mutagenesis Insertional Phenotype Genes Bacterial Mutation Food Microbiology Transposon mutagenesis Food Science Biotechnology |
| Popis: | Cronobacter spp. are opportunistic food-borne pathogens that are responsible for rare but highly fatal cases of meningitis and necrotizing enterocolitis in neonates. While the operon responsible for yellow pigmentation in Cronobacter sakazakii strain ES5 was described recently, the involvement of additional genes in pigment expression and the influence of pigmentation on the fitness of Cronobacter spp. have not been investigated. Thus, the aim of this study was to identify further genes involved in pigment expression in Cronobacter sakazakii ES5 and to assess the influence of pigmentation on growth and persistence under conditions of environmental stress. A knockout library was created using random transposon mutagenesis. The screening of 9,500 mutants for decreased pigment production identified 30 colorless mutants. The mapping of transposon insertion sites revealed insertions in not only the carotenoid operon but also in various other genes involved in signal transduction, inorganic ions, and energy metabolism. To determine the effect of pigmentation on fitness, colorless mutants (Δ crtE , Δ crtX , and Δ crtY ) were compared to the yellow wild type using growth and inactivation experiments, a macrophage assay, and a phenotype array. Among other findings, the colorless mutants grew at significantly increased rates under osmotic stress compared to that of the yellow wild type while showing increased susceptibility to desiccation. Moreover, Δ crtE and Δ crtY exhibited increased sensitivity to UVB irradiation. |
| Databáze: | OpenAIRE |
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