Size-dependent antirecombinogenic effect of short spacers on palindrome recombinogenicity
| Autor: | Ivan-Krešimir Svetec, Berislav Lisnić, Bojan Žunar, Marina Svetec Miklenić, Angela Matanović, Anamarija Štafa, Nikolina Gatalica |
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| Rok vydání: | 2019 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Flap Endonucleases Saccharomyces cerevisiae Biochemistry Genetic recombination 03 medical and health sciences Endonuclease chemistry.chemical_compound 0302 clinical medicine Quasipalindromes with short spacers Yeast Saccharomyces cerevisiae DNA Fungal Molecular Biology Palindromes in DNA 030304 developmental biology Palindromic sequence Genetics Recombination Genetic 0303 health sciences biology Base Sequence RecQ Helicases BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences Inverted Repeat Sequences Palindrome Helicase Cell Biology MUS81 chemistry 030220 oncology & carcinogenesis biology.protein BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti DNA Sgs1 |
| Zdroj: | DNA Repair Volume 90 |
| ISSN: | 1568-7856 1568-7864 |
| Popis: | Palindromic sequences in DNA can instigate genetic recombination and genome instability, which can result in devastating conditions such as the Emmanuel syndrome. Palindrome recombinogenicity increases with its size and sequence similarity between palindrome arms, while quasipalindromes with long spacers are less recombinogenic. However, the minimal spacer length, which could reduce or abolish palindrome recombinogenicity in the eukaryotic genome, was never determined. Therefore, we constructed a series of palindromes containing spacers of lengths ranging from 0 (perfect palindrome) to 10 bp and tested their recombinogenicity in yeast Saccharomyces cerevisiae. We found that a 7 bp spacer significantly reduces 126 bp palindrome recombinogenicity, while a 10 bp spacer completely stabilizes palindromes up to 150 bp long. Additionally, we showed that palindrome stimulated recombination rate is not dependent on Mus81 and Yen1 endonucleases. We also compared the recombinogenicity of a perfect 126 bp palindrome and a corresponding quasipalindrome consisting of the same palindrome arms with a stabilising 10 bp spacer in sgs1Δ and rad27Δ backgrounds, since both Sgs1 helicase and Rad27 endonuclease are implicated in preventing hairpin formation at palindromic sequences during replication. |
| Databáze: | OpenAIRE |
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