Epidemic Dissemination of a Carbapenem-ResistantAcinetobacter baumanniiClone CarryingarmATwo Years After Its First Isolation in an Italian Hospital
| Autor: | Lucilla Dolzani, Raffaela Bressan, Anna Knezevich, Cristina Lagatolla, Linda Furlanis, Annalisa Milan, Roberto Luzzati, Franca Cian, Enrico Angelo Tonin, Maria Luisa Deiana |
|---|---|
| Přispěvatelé: | Milan, Annalisa, Furlanis, Linda, Cian, Franca, Bressan, Raffaela, Luzzati, Roberto, Lagatolla, Cristina, Deiana, Maria Luisa, Knezevich, Anna, Tonin, ENRICO ANGELO, Dolzani, Lucilla |
| Rok vydání: | 2016 |
| Předmět: |
Acinetobacter baumannii
0301 basic medicine integron Clone (cell biology) Drug resistance Integron Polymerase Chain Reaction Disease Outbreaks Integrons Hospitals University carbapenemase Phylogeny Molecular Epidemiology biology Anti-Bacterial Agents ArmA OXA-23 outbreak Italy Acinetobacter Infections Plasmids Microbiology (medical) 030106 microbiology Immunology Microbiology beta-Lactam Resistance beta-Lactamases 03 medical and health sciences Bacterial Proteins Humans Alleles Retrospective Studies Pharmacology Molecular epidemiology Outbreak Gene Expression Regulation Bacterial Methyltransferases Sequence Analysis DNA biology.organism_classification Virology Clone Cells Carriage Carbapenems biology.protein Multilocus sequence typing Multilocus Sequence Typing |
| Zdroj: | Microbial Drug Resistance. 22:668-674 |
| ISSN: | 1931-8448 1076-6294 |
| DOI: | 10.1089/mdr.2015.0167 |
| Popis: | This study describes the dissemination of a carbapenem-resistant Acinetobacter baumannii (CRAB) strain in a university hospital in Northeast Italy. Characterization of the outbreak strain was combined with a retrospective analysis of all CRAB isolates collected in the same hospital during the 5 years preceding the outbreak, with the aim of elucidating the origin of the epidemic spread. The outbreak strain was shown to belong to the International Clone II and carry the blaOXA-23 gene, flanked by two ISAba1 sequences in opposite orientation (Tn2006 arrangement). The epidemic clone harbored also the blaOXA-66 allele of the carbapenemase intrinsic to A. baumannii, the determinant of ArmA 16S rRNA methylase and a class 1 integron, with the aacA4, catB8, and aadA1 cassette array. Genotype analysis, performed by macrorestriction analysis and VRBA, revealed that isolates related to outbreak strain had been sporadically collected from inpatients in the 2 years preceding outbreak start. Carriage of blaOXA-66, armA, and the integron further supported relatedness of these isolates to the outbreak clone. Outbreak initially involved three medical wards, typically hosting elderly patients with a history of prolonged hospitalization. The study highlights the need to adopt strict infection control measures also when CRAB isolation appears to be a sporadic event. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|