Spermatogonial Stem Cell Transplantation into Rhesus Testes Regenerates Spermatogenesis Producing Functional Sperm
| Autor: | Meena Sukhwani, Kyle E. Orwig, Julia N. Pascarella, Thea Ward, Felicity Winkler, Gerald Schatten, Cathy Ramsey, Shoukhrat Mitalipov, Angie Volk, Gina Dargo, Joseph E. Kiss, Mohamed Ezzelarab, Maura Lienesch, Brian P. Hermann, David K. C. Cooper, Yi Sheng, Kim Peterson, Angus W. Thomson, Hanna Valli, Maria Cecilia T. Penedo, Mario Rodriguez, Keith Masterson, Karen A. Peters |
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| Rok vydání: | 2012 |
| Předmět: |
Male
endocrine system medicine.medical_treatment Biology Intracytoplasmic sperm injection Male infertility Andrology 03 medical and health sciences 0302 clinical medicine Testis Genetics medicine Animals Blastocyst Spermatogenesis 030304 developmental biology 0303 health sciences 030219 obstetrics & reproductive medicine Embryo Cell Biology medicine.disease Macaca mulatta Spermatozoa Research Highlight Sperm Spermatogonia Transplantation medicine.anatomical_structure Immunology Molecular Medicine Stem cell Stem Cell Transplantation |
| Zdroj: | Cell Stem Cell. 11:715-726 |
| ISSN: | 1934-5909 |
| Popis: | Summary Spermatogonial stem cells (SSCs) maintain spermatogenesis throughout a man's life and may have application for treating some cases of male infertility, including those caused by chemotherapy before puberty. We performed autologous and allogeneic SSC transplantations into the testes of 18 adult and 5 prepubertal recipient macaques that were rendered infertile with alkylating chemotherapy. After autologous transplant, the donor genotype from lentivirus-marked SSCs was evident in the ejaculated sperm of 9/12 adult and 3/5 prepubertal recipients after they reached maturity. Allogeneic transplant led to donor-recipient chimerism in sperm from 2/6 adult recipients. Ejaculated sperm from one recipient transplanted with allogeneic donor SSCs were injected into 85 rhesus oocytes via intracytoplasmic sperm injection. Eighty-one oocytes were fertilized, producing embryos ranging from four-cell to blastocyst with donor paternal origin confirmed in 7/81 embryos. This demonstration of functional donor spermatogenesis following SSC transplantation in primates is an important milestone for informed clinical translation. |
| Databáze: | OpenAIRE |
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