No Factors Except for the Hap Complex increase the Taka-amylase A Gene Expression by Binding to the CCAAT Sequence in the Promoter Region
Autor: | Fumiko Naruse, Masashi Kato, Norihiro Tsukagoshi, Tetsuo Kobayashi |
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Rok vydání: | 2001 |
Předmět: |
Mutant
CAAT box complex mixtures Applied Microbiology and Biotechnology Biochemistry Aspergillus nidulans Gene Expression Regulation Enzymologic Analytical Chemistry Fungal Proteins Transformation Genetic stomatognathic system Transcription (biology) Gene Expression Regulation Fungal parasitic diseases Gene expression Promoter Regions Genetic Molecular Biology Gene Regulation of gene expression biology Organic Chemistry Promoter General Medicine biology.organism_classification Molecular biology digestive system diseases CCAAT-Binding Factor alpha-Amylases Biotechnology |
Zdroj: | Bioscience, Biotechnology, and Biochemistry. 65:2340-2342 |
ISSN: | 1347-6947 0916-8451 |
DOI: | 10.1271/bbb.65.2340 |
Popis: | To examine the possibility that factors different from the Hap complex are involved in increasing Taka-amylase A gene (taa) expression in Aspergillus nidulans, either the authentic taa gene or the mutant taa gene with a replacement of the CCAAT box was expressed in either a wild type strain or a mutant strain lacking the functional Hap complex (hapCdelta). When the mutant taa was expressed in the hapCdelta strain, enzyme activity was as low as that of the hapCdelta strain transformed with the authentic taa gene, indicating that no factors except for the Hap complex increase transcription of the taa gene by binding to the CCAAT sequence. |
Databáze: | OpenAIRE |
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