Agrobacterium tumefaciens-mediated transformation of chickpea (Cicer arietinum L.): gene integration, expression and inheritance
| Autor: | David S. Baliski, Patricia L. Polowick, John D. Mahon |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA
Plant Agrobacterium Recombinant Fusion Proteins Genetic Vectors Inheritance Patterns Plant Science Genetically modified crops Tissue culture Transformation Genetic Gene Expression Regulation Plant Botany Promoter Regions Genetic Gene Glucuronidase Regulation of gene expression biology food and beverages General Medicine Agrobacterium tumefaciens biology.organism_classification Plants Genetically Modified Molecular biology Cicer Transformation (genetics) Seeds Gene Fusion Agronomy and Crop Science Genome Plant Plant Shoots Explant culture Plasmids |
| Zdroj: | Plant cell reports. 23(7) |
| ISSN: | 0721-7714 |
| Popis: | A reproducible method of Agrobacterium-mediated transformation was developed for Cicer arietinum (chickpea). Initial explants consisted of longitudinal slices from embryonic axes of imbibed, mature seed. The plasmid contained a bi-functional fusion gene conferring both beta-glucuronidase and neomycin phosphotransferase activities, under the control of a 35S35SAMV promoter. Using a series of tissue culture media for co-cultivation, shoot initiation and rooting, we recovered transgenic plants from approximately 1.3% of the sliced embryo axes. The addition of a shoot elongation medium to the protocol improved the success rate to 3.1% but increased the time in tissue culture. Inheritance of the gus gene was followed through four generations, both through expression and Southern hybridization assays, and showed the expected Mendelian inheritance pattern. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink