Complete sequence of human T cell leukemia virus type 1 in ATLL patients from Northeast Iran, Mashhad revealed a prematurely terminated protease and an elongated pX open reading frame III
Autor: | Mehran Mohareri, Rohollah Arab, Seyed Abdolrahim Rezaee, Mohammad Mahdi Koshyar, Ali Mirhosseini, Abolghasem Allahyari, Baratali Mashkani, Hossein Rahimi, Ali Bazarbachi, Reza Boostani, Zahra Mozaheb, Alireza Bari, Abbas Shirdel, Houshang Rafatpanah |
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Rok vydání: | 2019 |
Předmět: |
Male
0301 basic medicine Microbiology (medical) Canada China Genes Viral Sequence analysis viruses 030106 microbiology Iran Biology Microbiology Genome DNA sequencing Virus Open Reading Frames 03 medical and health sciences Complete sequence Japan Germany hemic and lymphatic diseases Genetics Humans Leukemia-Lymphoma Adult T-Cell Viral Regulatory and Accessory Proteins Amino Acid Sequence Molecular Biology Genome size Ecology Evolution Behavior and Systematics Repetitive Sequences Nucleic Acid Whole genome sequencing Human T-lymphotropic virus 1 Base Sequence Nucleic acid sequence virus diseases Middle Aged Virology 030104 developmental biology Infectious Diseases DNA Viral Female France Brazil Peptide Hydrolases Transcription Factors |
Zdroj: | Infection, Genetics and Evolution. 73:460-469 |
ISSN: | 1567-1348 |
Popis: | To gain insight into the origin, evolution, dissemination and viral factors affecting HTLV-1-associated diseases, knowing the complete viral genome sequences is important. So far, no full-length HTLV-1 genome sequence has been reported from Iran. Here we report the complete nucleotide sequence of HTLV-1 viruses isolated from adult T cell leukemia/lymphoma (ATLL) patients from this region. The genome size of HTLV-1-MhD (Mashhad) was found to be 9036 bp and sequence analysis of the LTR region showed that it belongs to cosmopolitan subtype A. Comparing the sequences with isolates from another endemic area (HTLV-1ATK) revealed variations in the U3 region (~3.4%), while there was 99.1% and 97.0% similarity in R and U5 regions, respectively. The nucleotide sequences of HTLV-1 gag, pro and pol genes had a difference of 1.1% compared with HTLV-1 ATK with 16 nucleotides replaced in the gag and 27 in the pol regions. There was no variability in the amino acid sequences in the p24gag, however three residues were different in the p19gag and one in the p15gag. The nucleotide sequence of env showed a divergence of 1.5% compared to ATK with 22-nucleotide variation. The HTLV-1-MhD Tax, p13, p30, and p12 had 99.1, 100, 98.8, and 98%, respectively similarity with the prototype strain. Four amino acid changes were detected in ORF1 and ORF2 products p12 and p30, respectively, while the p13 region showed 100% conservation. The nucleotide identity between the isolates of Mashhad and those isolated from France, Germany, China, Canada and Brazil was 99.1%, 99.2%, 97.9%, 99% and 99.3%, respectively. Four amino acid changes compared with HTLV-1ATK from Japan were detected in ORF1 and ORF2 products p12 and p30, respectively, while the p13 region showed 100% conservation. This data could provide information regarding the evolutionary history, phylogeny, origin of the virus and vaccine design. |
Databáze: | OpenAIRE |
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