Nanometer-Scale Force Profiles of Short Single- and Double-Stranded DNA Molecules on a Gold Surface Measured Using a Surface Forces Apparatus
| Autor: | Kevin W. Plaxco, Jing Yu, Matthew Tirrell, Di Kang, Hongbo Zeng, Jun Huang, Fan Xia, Jacob N. Israelachvili |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Characteristic length Polymers DNA Single-Stranded Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Article Single-Stranded Monolayer Electrochemistry Molecule Nanotechnology General Materials Science Spectroscopy chemistry.chemical_classification Chemical Physics Rational design Surface forces apparatus Surfaces and Interfaces Polymer DNA 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Sphere packing chemistry Chemical physics Nanometre Gold 0210 nano-technology |
| Zdroj: | Langmuir Langmuir : the ACS journal of surfaces and colloids, vol 37, iss 45 |
| ISSN: | 1520-5827 |
| Popis: | Using a surface forces apparatus (SFA), we have studied the nanomechanical behavior of short single-stranded and partially and fully double-stranded DNA molecules attached via one end to a self-assembled monolayer on a gold surface. Our results confirm the previously proposed "mushroom-like" polymer structure for surface-attached, single-stranded DNA at low packing density and a "brush-like" structure for the same construct at higher density. At low density we observe a transition to "rigid rod" behavior upon addition of DNA complementary to the surface-attached single strand as the fraction of molecules that are double-stranded increases, with a concomitant increase in the SFA-observed thickness of the monolayer and the characteristic length of the observed repulsive forces. At higher densities, in contrast, this transition is effectively eliminated, presumably because the single-stranded state is already extended in its "brush" state. Taken together, these studies offer insights into the structure and physics of surface-attached short DNAs, providing new guidance for the rational design of DNA-modified functional surfaces. |
| Databáze: | OpenAIRE |
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