The optimized biocatalytic synthesis of ( S )‐methyl 2‐chlorobutanoate by Acinetobacter sp. lipase

Autor:	Yuele Lu, Rong Zhan, Beibei Song, Yaoyao Zhou, Linjiang Zhu, Hanchi Chen, Xiaolong Chen
Rok vydání:	2022
Předmět:	Pharmacology Levetiracetam Acinetobacter Hydrolysis Organic Chemistry Drug Discovery Biocatalysis Stereoisomerism Lipase Spectroscopy Catalysis Analytical Chemistry
Zdroj:	Chirality. 34:1228-1238
ISSN:	1520-636X 0899-0042
Popis:	Epilepsy is a chronic disease caused by sudden abnormal discharge of brain neurons, leading to transient brain dysfunction. Levetiracetam, developed by the UCB company in Belgium, is an effective drug for the treatment of epilepsy. (S)-Methyl 2-chlorobutanoate is an important chiral building block of levetiracetam, which has attracted a great deal of attention. In this study, a strain of lipase-produced Acinetobacter sp. zjutfet-1 was screened from soil samples. At optimized conditions for fermentation and biocatalysis, the bacterial lipase exhibited high catalytic activity for hydrolysis and stereoselectivity toward racemic methyl 2-chlorobutanoate. When the enzymatic reaction was carried out in 6% of racemic substrate, the enantiomeric excess (e.e.
Databáze:	OpenAIRE
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