IL-27 increases BST-2 expression in human monocytes and T cells independently of type I IFN
| Autor: | Masany Jung, Ashley Graveline, Bruce W. Banfield, Christina Guzzo, Katrina Gee |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
APOBEC
T-Lymphocytes Biology GPI-Linked Proteins Monocytes Article Cell Line Cytosine Deaminase 03 medical and health sciences 0302 clinical medicine Antigens CD Interferon Cytidine Deaminase medicine Humans APOBEC Deaminases RNA Messenger 030304 developmental biology 0303 health sciences Multidisciplinary Interleukin-17 Cytidine deaminase Flow Cytometry Molecular biology 3. Good health Interferon Type I Immunology Tetherin Cytokine secretion Interleukin 17 Signal transduction Interferon type I Signal Transduction 030215 immunology medicine.drug |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | IL-27 modulates inflammatory responses by influencing cytokine secretion and CD4 T cell differentiation. Recently, IL-27 was demonstrated to inhibit HIV replication by inducing type I interferon (IFN) expression and subsequent IFN-dependent expression of apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like (APOBEC)-3 family members, a group of antiviral cytidine deaminases. To characterize other anti-viral genes modulated by IL-27, we examined another IFN-responsive gene: tetherin/bone marrow stromal cell antigen 2 (BST-2). Our study shows that IL-27 can directly induce BST-2 expression, independently of an intermediary type I IFN response. Quantitative RT-PCR analysis demonstrated IL-27-induced BST-2 mRNA expression as early as 2h after exposure of cells to IL-27. In the presence of the type I IFN-neutralizing protein, B18R, IL-27-induced BST-2 expression was maintained, demonstrating that IFN is not an intermediary in IL-27-induced BST-2. Taken together, our findings identify a novel function of IL-27 as a direct stimulator of BST-2 expression. |
| Databáze: | OpenAIRE |
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