Increased expression of phospholipase D1 mRNA during cAMP- or NGF- induced differentiation in PC12 cells
| Autor: | Shigeru Nakashima, Kazuki Hayakawa, Yatsuji Ito, Keisuke Mizuta, Yoshinori Nozawa, Hideo Miyata |
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| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Neurite Cellular differentiation Phospholipase Biology PC12 Cells Internal medicine Cyclic AMP Neurites Phospholipase D medicine Animals Nerve Growth Factors RNA Messenger General Neuroscience Cell Differentiation Rats Cell biology Isoenzymes medicine.anatomical_structure Endocrinology Nerve growth factor Bucladesine nervous system Cell culture Neuron Phospholipase D1 |
| Zdroj: | Neuroscience Letters. 265:127-130 |
| ISSN: | 0304-3940 |
| Popis: | The rat pheochromocytoma PC12 cells have been employed as a useful model to study neuronal differentiation. To gain insight into the molecular events involved in extension of neurites, the differential expression of phospholipase D (PLD) isozymes were examined in cyclic AMP- or nerve growth factor (NGF)-treated PC12 cells. When the cells were incubated with dibutyryl cyclic AMP (dbcAMP), almost all the cells displayed prominent neurite outgrowth at 24 h. The neurites developed in NGF-treated cells at 24 h were shorter than those in the cells treated with dbcAMP. However, most of the NGF-treated cells extended long neurites at day 5. The rPLD1b mRNA increased within 6 h following dbcAMP treatment and maintained a high level up to 24 h. In contrast, the levels of rPLD1a and rPLD2 mRNAs were rather consistent throughout the time course examined. However, when the cells were treated with NGF, rPLD1a and rPLD1b mRNAs, but not rPLD2 mRNA, increased within 2 days and remained elevated up to 5 days. These results suggest the possible implication of PLD1 in PC12 cell differentiation. |
| Databáze: | OpenAIRE |
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