Development and implementation of a scalable and versatile test for COVID-19 diagnostics in rural communities

Autor: Robyn A. Umans, N. Bissell, Harald Sontheimer, F. M. Michel, Dipan C. Patel, Carmen Muñoz-Ballester, Carla V. Finkielstein, Oscar B. Alcoreza, Michael J. Friedlander, T. Maynard, P. Bordwine, Bhanu P. Tewari, Allison N. Tegge, A. Ceci, Daniel Martinez-Martinez, Joelle Martin, K. L. Brown
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Emergency Use Authorization
medicine.medical_specialty
Public land
Computer science
Science
Supply chain
General Physics and Astronomy
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
General Biochemistry
Genetics and Molecular Biology
Article
Specimen Handling
Genomic analysis
03 medical and health sciences
0302 clinical medicine
Limit of Detection
Nasopharynx
Pandemic
medicine
Humans
Pandemics
Health policy
Multidisciplinary
SARS-CoV-2
Public health
COVID-19
General Chemistry
Equipment Design
Gene expression profiling
Test (assessment)
Identification (information)
030104 developmental biology
Risk analysis (engineering)
COVID-19 Nucleic Acid Testing
Communicable Disease Control
Printing
Three-Dimensional
RNA
Viral
Reagent Kits
Diagnostic
Rural Health Services
Rural area
030217 neurology & neurosurgery
Zdroj: Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
ISSN: 2041-1723
Popis: Rapid and widespread testing of severe acute respiratory coronavirus 2 (SARS-CoV-2) is essential for an effective public health response aimed at containing and mitigating the coronavirus disease 2019 (COVID-19) pandemic. Successful health policy implementation relies on early identification of infected individuals and extensive contact tracing. However, rural communities, where resources for testing are sparse or simply absent, face distinctive challenges to achieving this success. Accordingly, we report the development of an academic, public land grant University laboratory-based detection assay for the identification of SARS-CoV-2 in samples from various clinical specimens that can be readily deployed in areas where access to testing is limited. The test, which is a quantitative reverse transcription polymerase chain reaction (RT-qPCR)-based procedure, was validated on samples provided by the state laboratory and submitted for FDA Emergency Use Authorization. Our test exhibits comparable sensitivity and exceeds specificity and inclusivity values compared to other molecular assays. Additionally, this test can be re-configured to meet supply chain shortages, modified for scale up demands, and is amenable to several clinical specimens. Test development also involved 3D engineering critical supplies and formulating a stable collection media that allowed samples to be transported for hours over a dispersed rural region without the need for a cold-chain. These two elements that were critical when shortages impacted testing and when personnel needed to reach areas that were geographically isolated from the testing center. Overall, using a robust, easy-to-adapt methodology, we show that an academic laboratory can supplement COVID-19 testing needs and help local health departments assess and manage outbreaks. This additional testing capacity is particularly germane for smaller cities and rural regions that would otherwise be unable to meet the testing demand.
Here, the authors report the development of a versatile academic, SARSCoV-2 RT-qPCR molecular diagnostic test that uses 3D printed technology for sample collection, is implemented in rural setting in the US state of Virginia and validated in its population.
Databáze: OpenAIRE
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