Real-time monitoring of reduced beta-adrenergic response in fibroblasts from patients with pseudohypoparathyroidism
| Autor: | Olivia C. Ong, Bernard K.-K. Fung, Cornelis Van Dop |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Cell signaling medicine.medical_treatment Biophysics Biology Biochemistry Computer Systems Internal medicine medicine Pseudohypoparathyroidism type Ia Humans Receptor Molecular Biology Pseudohypoparathyroidism Cells Cultured Monitoring Physiologic Insulin Isoproterenol β adrenergic Cell Biology Adrenergic beta-Agonists Fibroblasts Hydrogen-Ion Concentration medicine.disease Endocrinology Extracellular acidification Oxidation-Reduction Hormone Signal Transduction |
| Zdroj: | Analytical biochemistry. 238(1) |
| ISSN: | 0003-2697 |
| Popis: | The activation of cell-surface receptors usually produces a transient increase of the extracellular acidification rate in culture cells that can be detected with a biosensor-based instrument. We describe here the application of this method in monitoring hormonal response of Galphas-deficient fibroblasts from patients with pseudohypoparathyroidism type Ia (PHP-Ia). We found that following exposure to isoproterenol, the mean acidification response of fibroblasts from four PHP-Ia patients was only 18% of the response in normal cells. In contrast, these two groups of fibroblasts had similar levels of response to insulin and dibutyryl cAMP. This is the first reported experimental evidence correlating reduced Galphas activity with diminished cellular responsiveness to hormones in cultured living cells. Our results also indicate that this approach will be useful for rapid screening of other metabolic diseases caused by abnormal cellular signaling. |
| Databáze: | OpenAIRE |
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