HCV infective virions can be carried by human platelets
| Autor: | Agostino Pugliese, Emanuella Morra, Antonio Ponzetto, Miguel Angel Cutufia, Luisa Gennero, M. Enrietto, P. Pescarmona |
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| Rok vydání: | 2004 |
| Předmět: |
Blood Platelets
Hepatitis C virus Clinical Biochemistry Hepacivirus Biology medicine.disease_cause Immunofluorescence Biochemistry Cell Line Antigen fibronectin medicine Humans Platelet THP1 cell line Hepatitis HCV infection THP-1 cells platelets medicine.diagnostic_test Virion virus diseases Cell Biology General Medicine medicine.disease Virology Hepatitis C digestive system diseases Cell culture Hepatocellular carcinoma Immunology |
| Zdroj: | Cell biochemistry and function. 22(6) |
| ISSN: | 0263-6484 |
| Popis: | It has been previously demonstrated that platelets (PLTs) can bind and transport HIV-1 infectious virions. Hepatitis C virus (HCV)–HIV-1 co-infection occurs frequently among users of illicit intravenous drugs, thereby increasing the severity of HIV disease and the evolution towards chronic active hepatitis and hepatocellular carcinoma of HCV-related hepatitis. In the present study we investigated whether or not PLTs can carry HCV, and studied the binding mechanisms. Purified PLTs, obtained from healthy donors, HCV negative and HIV negative, were adsorbed with HCV-containing serum and then employed to infect a THP-1 monocytoid cell line. Replication of HCV was observed as shown by positivity for the E2 antigen within THP-1 cells, by indirect immunofluorescence; moreover, HCV-RNA was detected in supernatants of THP-1 cells at day 7 post-incubation with HCV-adsorbed PLTs. The binding of HCV to PLTs seems to involve fibronectin (FN), as already shown in the case of HIV-1. Indeed, treatment with RGD (Gly-Arg-Gly-Asp-Ser), the key oligopeptide of FN binding, inhibits the ability of HCV to be carried by PLTs in infective forms; the same phenomenon occurs with Mabs to FN. Moreover the infection of THP-1 cells seems to increase FN surface expression, as demonstrated by immunofluorescence tests. Copyright © 2004 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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