Suppressing effect of antimutagenic flavorings on chromosome aberrations induced by UV-light or X-rays in cultured Chinese hamster cells
| Autor: | Hisako Imanishi, YūF. Sasaki, Toshihiro Ohta, Mie Watanabe, Yasuhiko Shirasu |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
DNA polymerase
Cell Survival Ultraviolet Rays Health Toxicology and Mutagenesis Hamster Radiation-Protective Agents Chromosome aberration Chinese hamster chemistry.chemical_compound Cricetulus Cricetinae Genetics Animals Molecular Biology Interphase Cells Cultured Chromosome Aberrations biology Vanillin X-Rays Cell cycle biology.organism_classification Dideoxynucleosides Flavoring Agents chemistry Biochemistry Benzaldehydes biology.protein Antimutagen DNA |
| Zdroj: | Mutation research. 229(1) |
| ISSN: | 0027-5107 |
| Popis: | Chromosome aberrations induced by UV-light or X-rays were suppressed by the post-treatment with antimutagenic flavorings, such as anisaldehyde, cinnamaldehyde, coumarin, and vanillin. UV- or X-ray-irradiated surviving cells increased in the presence of each flavoring. X-ray-induced breakage-type and exchange-type chromosome aberrations were suppressed by the vanillin treatment in the G 1 phase of the cell cycle and a greater decrease in the number of X-ray-induced chromosome aberrations during G 1 holding was observed in the presence of vanillin. Furthermore, a greater decrease in the number of X-ray-induced DNA single-strand breaks was observed in the presence of vanillin. Treatment with vanillin in the G 2 phase suppressed UV- and X-ray-induced breakage-type but not exchange-type chromosome aberrations. The suppression of breakage-type aberrations was assumed to be due to a modification of the capability of the post-replicational repair of DNA double-strand breaks. These G 1 - and G 2 -dependent anticlastogenic effects were not observed in the presence of 2′,3′-dideoxythymidine, an inhibitor of DNA polymerase β. Based on these results, the anticlastogenic effect of vanillin was considered to be due to the promotion of the DNA rejoining process in which DNA polymerase β acts. |
| Databáze: | OpenAIRE |
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