Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012

Autor: Cecilia Olguin Perglione, Mariano Carossino, Agustina Rimondi, María Barrandeguy, Ann Cullinane, S. Miño, Sarah Gildea, A. Vissani
Rok vydání: 2015
Předmět:
0301 basic medicine
Pulmonary and Respiratory Medicine
040301 veterinary sciences
Epidemiology
viruses
América del Sur
Equine influenza
Argentina
Florida clade 1
Caballos
Enfermedades de los Animales
Biology
Real-Time Polymerase Chain Reaction
Virus
Animal Diseases
Virus de la Influenza Equina
Serology
Disease Outbreaks
0403 veterinary science
03 medical and health sciences
Influenza A Virus
H3N8 Subtype
Orthomyxoviridae Infections
Phylogenetics
Nasopharynx
equine influenza
Epidemiología
Animals
Horses
Clade
Gene
Phylogeny
Phylogenetic tree
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis
RNA
Public Health
Environmental and Occupational Health
Outbreak
Equine Influenzavirus
04 agricultural and veterinary sciences
Original Articles
Virology
030104 developmental biology
Infectious Diseases
Uruguay
Horse Diseases
Original Article
Zdroj: Influenza and Other Respiratory Viruses
Influenza and Other Respiratory Viruses 10 (1) : 37–46 (January 2016)
INTA Digital (INTA)
Instituto Nacional de Tecnología Agropecuaria
instacron:INTA
ISSN: 1750-2659
Popis: Background: In 2012, equine influenza (EI) virus was confirmed as the cause of outbreaks of respiratory disease in horses throughout South America. In Uruguay and Argentina, hundreds of vaccinated thoroughbred horses in training and racing facilities were clinically affected. Objective: To characterise the EI viruses detected during the outbreak in Uruguay and Argentina. Methods: Virus was detected in nasopharyngeal swabs by a pan-reactive influenza type A real-time RT-PCR. The nucleotide sequence of the HA1 gene was determined and analysed phylogenetically using mega 5 software. Amino acid sequences alignments were constructed and virus was antigenically characterised with specific ferret antisera. Paired serum samples were tested by haemagglutination inhibition and single radial haemolysis. Results: The diagnosis of EIV was confirmed by real-time RT-PCR, virus isolation and serological testing. The phylogenetic analysis of HA1 gene sequences of 18 EI viruses indicated that all of them belong to clade 1 of the Florida sublineage of the American lineage and are closely related to viruses isolated in the United States in 2012. The HA1 of viruses identified in horses in racing facilities in Maroñas, Uruguay, and in Palermo, Argentina, displayed 100% amino acid sequence identity and were identical to that of a virus isolated in Dubai in 2012, from vaccinated endurance horses recently imported from Uruguay. Conclusions: The surveillance data reported illustrate the international spread of EI viruses and support the recommendations of the OIE expert surveillance panel to include viruses of the Florida sublineage in vaccines. Fil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Gildea, Sarah. The Irish Equine Centre. Virology Unit; Irlanda Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Carossino, Mariano. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos Fil: Cullinane, Ann. The Irish Equine Centre. Virology Unit; Irlanda Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina
Databáze: OpenAIRE
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