Distribution of Bartonella henselae variants in patients, reservoir hosts and vectors in Spain
| Autor: | Cristina García-Amil, Ferran Segura, Ramón A. Juste, Coral García-Esteban, F. Valcárcel, Raquel Escudero, Horacio Gil, Isabel Rodríguez-Moreno, Santos Jiménez, Pedro Anda, Bruno Lobo, Isabel Jado, Manuela Rodríguez-Vargas, Azucena Pérez, Inmaculada Pons |
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| Přispěvatelé: | Instituto de Salud Carlos III |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Evolutionary Genetics
Bartonella Epidemiology lcsh:Medicine Minisatellite Repeats Disease Vectors Multiple Loci VNTR Analysis Microbiology Microbial Ecology Bartonella Infections medicine Animals Humans Evolutionary Systematics Typing lcsh:Science Clade Biology Phylogeny Disease Reservoirs Genetics Evolutionary Biology Bacterial Evolution Bartonella henselae Multidisciplinary Population Biology biology lcsh:R Cat-Scratch Disease Bacteriology Cat-scratch disease biology.organism_classification medicine.disease Virology Bacterial Pathogens Phylogenetics Phylogeography Variable number tandem repeat Emerging Infectious Diseases Spain Microbial Evolution Cats Multilocus sequence typing lcsh:Q Ctenocephalides Research Article Multilocus Sequence Typing |
| Zdroj: | Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA PLoS ONE PLoS ONE, Vol 8, Iss 7, p e68248 (2013) INIA: Repositorio de Resultados de Investigación del INIA Repisalud Instituto de Salud Carlos III (ISCIII) |
| Popis: | We have studied the diversity of B. henselae circulating in patients, reservoir hosts and vectors in Spain. In total, we have fully characterized 53 clinical samples from 46 patients, as well as 78 B. henselae isolates obtained from 35 cats from La Rioja and Catalonia (northeastern Spain), four positive cat blood samples from which no isolates were obtained, and three positive fleas by Multiple Locus Sequence Typing and Multiple Locus Variable Number Tandem Repeats Analysis. This study represents the largest series of human cases characterized with these methods, with 10 different sequence types and 41 MLVA profiles. Two of the sequence types and 35 of the profiles were not described previously. Most of the B. henselae variants belonged to ST5. Also, we have identified a common profile (72) which is well distributed in Spain and was found to persist over time. Indeed, this profile seems to be the origin from which most of the variants identified in this study have been generated. In addition, ST5, ST6 and ST9 were found associated with felines, whereas ST1, ST5 and ST8 were the most frequent sequence types found infecting humans. Interestingly, some of the feline associated variants never found on patients were located in a separate clade, which could represent a group of strains less pathogenic for humans. This work was supported by the Spanish Fondo de Investigación Sanitaria (PI10/00051 and PI10/00165). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Sí |
| Databáze: | OpenAIRE |
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