Synthesis of Poly(Malic Acid) Derivatives End-Functionalized with Peptides and Preparation of Biocompatible Nanoparticles to Target Hepatoma Cells
| Autor: | Catherine Ribault, Elise Vene, Clarisse Brossard, Nicolas Lepareur, Pascal Loyer, Manuel Vlach, Nicolas Noiret, Sandrine Cammas-Marion, Vincent Dorcet |
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| Přispěvatelé: | Synthèse Caractérisation Analyse de la Matière (ScanMAT), Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut de Chimie du CNRS (INC), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CHU Pontchaillou [Rennes], This work was funded by the Fondation pour la Recherche Médicale (FRM N°DCM20181039540), Cancéropôle Grand Ouest (CGO n° 17/028), Labex IRON (n° ANR-11-LABX-0018), Institut National de la Santé et de la Recherche Médicale (Inserm), l’Ecole Nationale Supérieure de Chimie de Rennes (ENSCR, France), the Centre National de la Recherche Scientifique (CNRS). Clarisse Brossard was recipient of a PhD fellowship from the Fondation pour la Recherche Médicale (FRM N°DCM20181039540)., ANR-11-LABX-0018,IRON,Radiopharmaceutiques Innovants en Oncologie et Neurologie(2011), Université de Rennes (UR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), HAL UR1, Admin, Radiopharmaceutiques Innovants en Oncologie et Neurologie - - IRON2011 - ANR-11-LABX-0018 - LABX - VALID |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
human hepatoma cells
General Chemical Engineering Nanoparticle Context (language use) Peptide circumsporozoite protein of Plasmodium berghei 02 engineering and technology 010402 general chemistry 01 natural sciences Article GB virus A lcsh:Chemistry chemistry.chemical_compound Dynamic light scattering [CHIM] Chemical Sciences [CHIM]Chemical Sciences General Materials Science Maleimide chemistry.chemical_classification poly(malic acid) derivatives (Co)polymers end-functionalized with peptide 021001 nanoscience & nanotechnology Combinatorial chemistry 0104 chemical sciences multifunctional nanoparticles chemistry lcsh:QD1-999 Drug delivery Electrophoretic light scattering 0210 nano-technology Ethylene glycol |
| Zdroj: | Nanomaterials Nanomaterials, MDPI, 2021, 11 (4), pp.958. ⟨10.3390/nano11040958⟩ Nanomaterials, 2021, 11 (4), pp.958. ⟨10.3390/nano11040958⟩ Nanomaterials, Vol 11, Iss 958, p 958 (2021) Volume 11 Issue 4 |
| ISSN: | 2079-4991 |
| Popis: | International audience; Recently, short synthetic peptides have gained interest as targeting agents in the design of site-specific nanomedicines. In this context, our work aimed at developing new tools for the diagnosis and/or therapy of hepatocellular carcinoma (HCC) by grafting the hepatotropic George Baker (GB) virus A (GBVA10-9) and Plasmodium circumsporozoite protein (CPB)-derived peptides to the biocompatible poly(benzyl malate), PMLABe. We successfully synthesized PMLABe derivatives end-functionalized with peptides GBVA10-9, CPB, and their corresponding scrambled peptides through a thiol/maleimide reaction. The corresponding nanoparticles (NPs), varying by the nature of the peptide (GBVA10-9, CPB, and their scrambled peptides) and the absence or presence of poly(ethylene glycol) were also successfully formulated using nanoprecipitation technique. NPs were further characterized by dynamic light scattering (DLS), electrophoretic light scattering (ELS) and transmission electron microscopy (TEM), highlighting a diameter lower than 150 nm, a negative surface charge, and a more or less spherical shape. Moreover, a fluorescent probe (DiD Oil) has been encapsulated during the nanoprecipitation process. Finally, preliminary in vitro internalisation assays using HepaRG hepatoma cells demonstrated that CPB peptide-functionalized PMLABe NPs were efficiently internalized by endocytosis, and that such nanoobjects may be promising drug delivery systems for the theranostics of HCC. |
| Databáze: | OpenAIRE |
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