Proteolytic systems’ expression during myogenesis and transcriptional regulation by amino acids in gilthead sea bream cultured muscle cells

Autor: Emilio J. Vélez, Dorothy Verheyden, Isabel Navarro, Albert Sánchez-Moya, Encarnación Capilla, Joaquim Gutiérrez, Sheida Azizi, Cristina Salmerón, Esmail Lutfi
Přispěvatelé: Universitat de Barcelona
Rok vydání: 2017
Předmět:
0301 basic medicine
Transcription
Genetic
Protein Expression
Muscle cells
Gene Expression
Muscle Proteins
lcsh:Medicine
Cathepsin D
Miogènesi
Muscle Development
Biochemistry
Myoblasts
0302 clinical medicine
Ubiquitin
Animal Cells
Cèl·lules musculars
Gene expression
Medicine and Health Sciences
Morphogenesis
Transcriptional regulation
Amino Acids
lcsh:Science
Cells
Cultured
Multidisciplinary
biology
Organic Compounds
Chemistry
Myogenesis
Stem Cells
Muscles
Muscle Differentiation
Cell biology
Physical Sciences
Cellular Types
Anatomy
Basic Amino Acids
Research Article
Muscle Tissue
Research and Analysis Methods
Real-Time Polymerase Chain Reaction
03 medical and health sciences
Leucine
Genetics
Gene Expression and Vector Techniques
Animals
Molecular Biology Techniques
Molecular Biology
Cathepsin
Muscle Cells
Molecular Biology Assays and Analysis Techniques
Lysine
Organic Chemistry
lcsh:R
Chemical Compounds
Protein turnover
Biology and Life Sciences
Proteins
Cell Biology
Expressió gènica
Sea Bream
Biological Tissue
030104 developmental biology
Aliphatic Amino Acids
Gene Expression Regulation
Proteasome
Proteolysis
biology.protein
lcsh:Q
Proteïnes
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Dipòsit Digital de la UB
Universidad de Barcelona
Recercat. Dipósit de la Recerca de Catalunya
instname
PLoS ONE, Vol 12, Iss 12, p e0187339 (2017)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0187339
Popis: Proteolytic systems exert an important role in vertebrate muscle controlling protein turnover, recycling of amino acids (AA) or its use for energy production, as well as other functions like myogenesis. In fish, proteolytic systems are crucial for the relatively high muscle somatic index they possess, and because protein is the most important dietary component. Thus in this study, the molecular profile of proteolytic markers (calpains, cathepsins and ubiquitin-proteasome system (UbP) members) were analyzed during gilthead sea bream (Sparus aurata) myogenesis in vitro and under different AA treatments. The gene expression of calpains (capn1, capn3 and capns1b) decreased progressively during myogenesis together with the proteasome member n3; whereas capn2, capns1a, capns1b and ubiquitin (ub) remained stable. Contrarily, the cathepsin D (ctsd) paralogs and E3 ubiquitin ligases mafbx and murf1, showed a significant peak in gene expression at day 8 of culture that slightly decreased afterwards. Moreover, the protein expression analyzed for selected molecules presented in general the same profile of the mRNA levels, which was confirmed by correlation analysis. These data suggest that calpains seem to be more important during proliferation, while cathepsins and the UbP system appear to be required for myogenic differentiation. Concerning the transcriptional regulation by AA, the recovery of their levels after a short starvation period did not show effects on cathepsins expression, whereas it down-regulated the expression of capn3, capns1b, mafbx, murf1 and up-regulated n3. With regards to AA deficiencies, the major changes occurred at day 2, when leucine limitation suppressed ctsb and ctsl expression. Besides at the same time, both leucine and lysine deficiencies increased the expression of mafbx and murf1 and decreased that of n3. Overall, the opposite nutritional regulation observed, especially for the UbP members, points out an efficient and complementary role of these factors that could be useful in gilthead sea bream diets optimization.
Databáze: OpenAIRE
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