Cigarette smoke extract combined with LPS down-regulates the expression of MRP2 in chronic pulmonary inflammation may be related to FXR
| Autor: | Zihao Wang, Chuanzong Qi, Shuyi Zhang, Jue Song, Xin Fang, Jian Zhou |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Immunology Down-Regulation Receptors Cytoplasmic and Nuclear Inflammation Pharmacology Cigarette Smoking Alveolar cells Rats Sprague-Dawley chemistry.chemical_compound Pulmonary Disease Chronic Obstructive In vivo Cell Line Tumor Smoke Gene silencing Medicine Animals Humans Molecular Biology Lung business.industry Multidrug resistance-associated protein 2 NF-kappa B Transporter Pneumonia Tobacco Products In vitro Multidrug Resistance-Associated Protein 2 Rats Up-Regulation medicine.anatomical_structure chemistry A549 Cells medicine.symptom Inflammation Mediators Multidrug Resistance-Associated Proteins business Signal Transduction |
| Zdroj: | Molecular immunology. 137 |
| ISSN: | 1872-9142 |
| Popis: | The transporter multidrug resistance protein 2 (MRP2) plays an important role in chronic pulmonary inflammation by transporting cigarette smoke and other related inflammatory mediators. However, it is not completely clear whether pulmonary inflammation caused by cigarette smoke extract (CSE) and lipopolysaccharide (LPS) is related to MRP2 and its signal factors. In this study, CSE combined with LPS was used to establish an inflammation model in vivo and in vitro. We found that compared with the control group, after CSE combined with LPS treatment, the expression of MRP2 in rat lung tissue in vivo and human alveolar cell line in vitro was down-regulated, while the expression of inflammatory factors was up-regulated. Through silencing and overexpression of FXR, it was found that silent FXR could down-regulate MRP2 and up-regulate the expression of inflammatory factors. On the contrary, overexpression of FXR could up-regulate MRP2 and down-regulate the expression of inflammatory factors. Our results show that CSE combined with LPS can down-regulate the expression of MRP2 under inflammatory conditions, and the down-regulation of MRP2 expression may be achieved partly through the FXR signal pathway. |
| Databáze: | OpenAIRE |
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