CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface
| Autor: | Georges Bismuth, Mark G.H. Scott, Catherine Labbé-Jullié, Lamia Achour, Hamasseh Shirvani, Stefano Marullo, Alain Thuret |
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| Přispěvatelé: | Marullo, Stefano, Institut Cochin (UMR_S567 / UMR 8104), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2009 |
| Předmět: |
MESH : Hela Cells
Chemokine receptor CCR5 viruses Receptor expression MESH: Antigens CD4 Intracellular Space MESH : Intracellular Space MESH: Cricetinae Plasma protein binding MESH: Receptors CCR5 Endoplasmic Reticulum Biochemistry MESH: Cell Compartmentation MESH : Protein Transport MESH: Cricetulus Cell–cell interaction Cricetinae MESH : CHO Cells MESH: Animals Receptor Cells Cultured MESH : Endoplasmic Reticulum biology MESH : Cricetinae virus diseases MESH : Protein Binding MESH : Receptors CCR5 Hematology Cell biology Protein Transport Antigens Surface CD4 Antigens MESH: Intracellular Space MESH : Antigens Surface Intracellular MESH: Cells Cultured Protein Binding MESH: Protein Transport Receptors CCR5 MESH: Antigens Surface MESH : Cricetulus Immunology [SDV.BC]Life Sciences [q-bio]/Cellular Biology CHO Cells Article Cricetulus MESH: CHO Cells MESH: Endoplasmic Reticulum MESH : Cells Cultured MESH: Protein Binding Animals Humans MESH : Cell Compartmentation [SDV.BC] Life Sciences [q-bio]/Cellular Biology MESH: Humans Endoplasmic reticulum MESH : Humans Cell Biology Cell Compartmentation MESH: Hela Cells biology.protein MESH : Antigens CD4 MESH : Animals CC chemokine receptors HeLa Cells |
| Zdroj: | Blood Blood, 2009, 113 (9), pp.1938-47. ⟨10.1182/blood-2008-02-141275⟩ Blood, American Society of Hematology, 2009, 113 (9), pp.1938-47. 〈10.1182/blood-2008-02-141275〉 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2008-02-141275 |
| Popis: | International audience; The association of CD4, a glycoprotein involved in T-cell development and antigen recognition, and CC chemokine receptor 5 (CCR5), a chemotactic G protein-coupled receptor, which regulates trafficking and effector functions of immune cells, forms the main receptor for HIV. We observed that the majority of CCR5 is maintained within the intracellular compartments of primary T lymphocytes and in a monocytic cell line, contrasting with its relatively low density at the cell surface. The CCR5-CD4 association, which occurs in the endoplasmic reticulum, enhanced CCR5 export to the plasma membrane in a concentration-dependent manner, whereas inhibition of endogenous CD4 with small interfering RNAs decreased cell-surface expression of endogenous CCR5. This effect was specific for CCR5, as CD4 did not affect cellular distribution of CXCR4, the other HIV coreceptor. These results reveal a previously unappreciated role of CD4, which contributes to regulating CCR5 export to the plasma membrane. |
| Databáze: | OpenAIRE |
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