Redox activity ofHydrodictyon reticulatum plasmalemma vesicles

Autor:	K. Janáček, J. Matoušková, R. Rybová, L. Nešpůrková
Rok vydání:	1999
Předmět:	chemistry.chemical_classification Chromatography Aqueous solution Vesicle General Medicine Polymer Chlorophyta Biology biology.organism_classification Microbiology Transmembrane protein Redox Activity chemistry Microsome Homogenization (biology)
Zdroj:	Folia Microbiologica. 44:419-424
ISSN:	1874-9356 0015-5632
DOI:	10.1007/bf02903716
Popis:	Plasmalemma vesicles with preserved redox activity were prepared from nets ofHydrodictyon reticulatum. Since the walls are mechanically very resistant, a combination of partial cell-wall enzyme digestion and ultrasonic homogenization had to be used for the disruption of cells. To isolate the plasma-membrane-enriched microsomal fraction separation in an aqueous two-phase polymer system was found to be most suitable. The right-side-out vesicles reduced added hexacyanoferrate(III) by electrons supplied either by a transmembrane flow or from external NADH.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6276f92d4c6b288a42489f5cb16bd7c https://doi.org/10.1007/bf02903716 Zobrazit plný text záznamu Full text from SpringerLink