Frequency analysis of the antibody specificity repertoire of mitogen-reactive B cells and 'spontaneously' occurring 'background' plaque-forming cells in nude mice
| Autor: | Herbert Hooijkaas, Aria A. van der Linde-Preesman, Robbert Benner, Sinka Benne |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide Immunology Mice Nude Bone Marrow Cells Hemolytic Plaque Technique chemistry.chemical_compound Epitopes Leukocyte Count Mice Antibody Specificity medicine Animals B cell B-Lymphocytes biology Repertoire Horse Molecular biology Rats Mice Inbred C57BL medicine.anatomical_structure chemistry Immunoglobulin M Rats Inbred Lew Mitogen-activated protein kinase biology.protein Female Bone marrow Antibody Clone (B-cell biology) Spleen |
| Zdroj: | Cellular immunology. 92(1) |
| ISSN: | 0008-8749 |
| Popis: | The antibody specificity repertoire of lipopolysaccharide (LPS)-reactive B cells has been determined in the spleens and bone marrow (BM) of C57BL/Ka athymic nude mice using a limiting dilution culture system that allows the growth and development of every LPS-reactive B cell into a clone of IgM-secreting cells. In addition, the numbers of “spontaneously” occurring (“background”) IgM-, IgG-, and IgA-secreting cells as well as the “background” IgM antibody specificity repertoire has been assessed in spleens and BM. The frequencies of antigen-specific LPS-reactive B cells of C57BL/Ka nude and thymus-bearing mice showed a great similarity and ranged from 1 in 1000 to 1 in 2500 for sheep red blood cells (SRBC), horse red blood cells (HRBC), and goat red blood cells (GRBC), from 1 in 10 to 1 in 25 for 5-iodo-3-nitrophenyl-coupled (SRBC), from 1 in 15 to 1 in 150 for 4-hydroxy-3,5-dinitrophenyl-coupled SRBC, and from 1 in 70 to 1 in 140 for 2,4,6-trinitrophenyl-coupled SRBC. The specificity repertoire of the “background” IgM-secreting cells differed from that of age-matched thymus-bearing controls and was different in young and old C57BL/Ka nude mice. Within the limitations of having assessed only a minor fraction of the total B-cell antibody specificity repertoire and supposing that nude mice are largely devoid of functional T cells, the data presented suggest that the generation of the specificity repertoire of newly-formed B cells is hardly or not affected by T cells. On the other hand, T cells do affect the expression of the established repertoire, represented by “background” immunoglobulin-secreting cells. |
| Databáze: | OpenAIRE |
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