Encapsulation of Protein-Polysaccharide HIP Complex in Polymeric Nanoparticles
| Autor: | Ashwin Parenky, Ripal Gaudana, Ashim K. Mitra, Varun Khurana |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Circular dichroism Article Subject biology lcsh:RS1-441 Ionic bonding Nanoparticle Polymer Bioinformatics lcsh:Pharmacy and materia medica PLGA chemistry.chemical_compound chemistry biology.protein Bovine serum albumin Fourier transform infrared spectroscopy Research Article Nuclear chemistry Macromolecule |
| Zdroj: | Journal of Drug Delivery Journal of Drug Delivery, Vol 2011 (2011) |
| ISSN: | 2090-3022 2090-3014 |
| Popis: | The objective of the present study is to formulate and characterize a nanoparticulate-based formulation of a macromolecule in a hydrophobic ion pairing (HIP) complex form. So far, HIP complexation approach has been studied only for proteins with molecular weight of 10–20 kDa. Hence, we have selected bovine serum albumin (BSA) having higher molecular weight (66.3 kDa) as a model protein and dextran sulphate (DS) as a complexing polymer to generate HIP complex. We have prepared and optimized the HIP complex formation process of BSA with DS. Ionic interactions between basic amino acids of BSA with sulphate groups of DS were confirmed by FTIR analysis. Further, nanoparticles were prepared and characterized with respect to size and surface morphology. We observed significant entrapment of BSA in nanoparticles prepared with minimal amounts of PLGA polymer. Finally, results of circular dichroism and intrinsic fluorescence assay have clearly indicated that HIP complexation and method of nanoparticle preparation did not alter the secondary and tertiary structures of BSA. |
| Databáze: | OpenAIRE |
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