Effect of Pterocarpus santalinus bark, on blood glucose, serum lipids, plasma insulin and hepatic carbohydrate metabolic enzymes in streptozotocin-induced diabetic rats
| Autor: | Chippada Appa Rao, Shaik Sameena Fatima, Sampath Kumar Mekala Thur, Kameswara Rao Badri, Dilip Rajasekhar Maddirala, Vinay Kumar Kondeti, Ramesh B. Kasetti |
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| Rok vydání: | 2010 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Pterocarpus medicine.medical_treatment Blood sugar Blood lipids Hyperlipidemias Chemical Fractionation Biology Toxicology Diabetes Mellitus Experimental chemistry.chemical_compound Phenols Internal medicine Diabetes mellitus Blood plasma medicine Animals Hypoglycemic Agents Insulin Glycosides Rats Wistar Flavonoids Glycogen Plant Extracts General Medicine medicine.disease Lipids Fructose-Bisphosphatase Liver Glycogen Rats Endocrinology Liver Gluconeogenesis chemistry Glucose-6-Phosphatase biology.protein Glucose 6-phosphatase Food Science |
| Zdroj: | Food and Chemical Toxicology. 48:1281-1287 |
| ISSN: | 0278-6915 |
| Popis: | Aim of the study The present study was designed to investigate the effect of bark of Pterocarpus santalinus , an ethnomedicinal plant, on blood glucose, plasma insulin, serum lipids and the activities of hepatic glucose metabolizing enzymes in streptozotocin-induced diabetic rats. Materials and methods Streptozotocin-induced diabetic rats were treated (acute/short-term and long-term) with ethyl acetate:methanol fractions of ethanolic extract of the bark of P . santalinus . Fasting blood glucose, HbA 1C , plasma insulin and protein were estimated before and after the treatment, along with hepatic glycogen, and activities of hexokinase, glucose-6-phosphatase, fructose-1,6-bisphosphatase and glucose-6-phosphate dehydrogenase. Further anti-hyperlipidemic activity was studied by measuring the levels of serum lipids and lipoproteins. Results Phytochemical analysis of active fraction showed the presence of flavonoids, glycosides and phenols. Biological testing of the active fraction demonstrated a significant antidiabetic activity by reducing the elevated blood glucose levels and glycosylated hemoglobin, improving hyperlipidemia and restoring the insulin levels in treated experimental induced diabetic rats. Further elucidation of mechanism of action showed improvement in the hepatic carbohydrate metabolizing enzymes after the treatment. Our present investigation suggests that active fraction of ethanolic extract of bark of P . santalinus decreases streptozotocin induced hyperglycemia by increasing glycolysis and decreasing gluconeogenesis. |
| Databáze: | OpenAIRE |
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