RNA sequencing reveals pronounced changes in the noncoding transcriptome of aging synaptosomes
| Autor: | Mara Knobloch, Nicola Bliim, Konii Takenaka, Jana Bochmann, Thomas Arendt, James D. Mills, E. Kirazov, Ludmil Kirazov, Uwe Ueberham, Renate Jendrek, Bei Jun Chen, Michael Janitz |
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| Přispěvatelé: | Other departments |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Aging RNA Untranslated Polyadenylation RNA Splicing Gene Expression RNA-Seq Biology Hippocampus Synaptic Transmission Transcriptome 03 medical and health sciences Cognition Gene expression Transcriptional regulation Animals RNA Messenger Genetics Messenger RNA Sequence Analysis RNA General Neuroscience Phosphotransferases RNA Brain Nuclear Proteins Cell biology Mice Inbred C57BL 030104 developmental biology RNA splicing RNA Long Noncoding Neurology (clinical) Geriatrics and Gerontology Developmental Biology Synaptosomes Transcription Factors |
| Zdroj: | Neurobiology of aging, 56, 67-77. Elsevier Inc. |
| ISSN: | 1558-1497 0197-4580 |
| Popis: | Normal aging is associated with impairments in cognitive functions. These alterations are caused by diminutive changes in the biology of synapses, and ineffective neurotransmission, rather than loss of neurons. Hitherto, only a few studies, exploring molecular mechanisms of healthy brain aging in higher vertebrates, utilized synaptosomal fractions to survey local changes in aging-related transcriptome dynamics. Here we present, for the first time, a comparative analysis of the synaptosomes transcriptome in the aging mouse brain using RNA sequencing. Our results show changes in the expression of genes contributing to biological pathways related to neurite guidance, synaptosomal physiology, and RNA splicing. More intriguingly, we also discovered alterations in the expression of thousands of novel, unannotated lincRNAs during aging. Further, detailed characterization of the cleavage and polyadenylation factor I subunit 1 (Clp1) mRNA and protein expression indicates its increased expression in neuronal processes of hippocampal stratum radiatum in aging mice. Together, our study uncovers a new layer of transcriptional regulation which is targeted by aging within the local environment of interconnecting neuronal cells. |
| Databáze: | OpenAIRE |
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